Cytoprotective effects of ferritin on doxorubicin-induced breast cancer cell death

Oncology Reports
Benjaporn Buranrat, James R Connor

Abstract

Ferritin is a major iron storage protein and essential for iron homeostasis. It has a wide range of functions in the body including iron delivery, immunosuppression, angiogenesis, and cell proliferation. Ferritin is overexpressed in many cancer cells, but its precise role in cancer is unclear. In the present study, we examined the functional roles of ferritin in protecting the MCF-7 breast cancer cell line against treatment with the chemotherapeutic agent doxorubicin. The effects of ferritin (human liver ferritin) and doxorubicin on the human MCF-7 breast cancer cell line were evaluated using the cell viability assay. The impact of decreasing ferritin light chain (FTL) and ferritin heavy chain (FTH) expression on doxorubicin sensitivity was assessed using siRNA. Reactive oxygen species (ROS) was also measured using the fluorescence probe CM-H2DCFDA. The mechanism of modulated chemosensitivity was evaluated by western blot analysis. Ferritin treatment activated MCF-7 cell proliferation in a concentration- and time-dependent manner. While treatment with doxorubicin alone significantly increased intracelullar ROS production, the addition of ferritin decreased this ROS formation, thereby reducing doxorubicin‑induced MCF-7 cell deat...Continue Reading

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Citations

Oct 17, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Carolina PanisEliana Abdelhay
Mar 20, 2014·Journal of Occupational Medicine and Toxicology·Yao-Peng HsiehChia-Chu Chang
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Nov 13, 2020·Technology in Cancer Research & Treatment·Yi GaoShuyu Zhang
Dec 10, 2020·Biomarker Research·Zhaoqing LiJichun Zhou
Mar 12, 2021·Frontiers in Oncology·Christian Récher
Apr 22, 2021·Metallomics : Integrated Biometal Science·Marina PlaysRaphaël Rodriguez

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Methods Mentioned

BETA
Assay
transfection
Protein Assay
electrophoresis
surgical resection

Software Mentioned

Multi Gauge

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