Cytoprotective effects of silymarin on epithelial cells against arsenic-induced apoptosis in contrast with quercetin cytotoxicity

Life Sciences
Elio A SoriaGuillermina A Bongiovanni

Abstract

To establish the differential cytoprotective activity against arsenic (As) toxicity of the flavonoids silymarin (S), which is without protective effects on cancer cells, and quercetin (Q). Arsenic (As) has a paradoxical biomedical role: it causes oxidative damage to normal cells leading to death or malignant transformation, but can be used, for the same reason, as an anticancer pro-apoptotic agent at high doses. Aqueous hydroperoxides (AHP), JNK (c-Jun N-terminal kinase) activation, caspase activity and death phenotype were assessed in CHO-K1 cells treated with As, S, Q, As+S and As+Q (p<0.05). Q proved to be toxic and did not exert complete protection against As, and only S was able to protect cells from As-induced oxidative death, which started after 4h with caspase activation and phosphatidylserine exteriorization on the outer cellular membrane. Although both flavonoids counteracted As-induced JNK activation (an early stress response, i.e. exposure biomarker), AHP were increased by As and Q (p<0.05). Moreover, Q-treated cells triggered per se apoptosis and even necrosis. Also, the classical sequence oxidative stress-JNK activation-cell death was seen for As, but not for Q, with S stopping the sequence. These results strongly...Continue Reading

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Citations

Jan 24, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Celia Fernández-BlancoMaria-Jose Ruiz
Jul 24, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H MalekinejadS Varasteh
Mar 22, 2012·Biochimica Et Biophysica Acta·Nicola SassiMario Zoratti
Jul 9, 2014·Advances in Pharmacological Sciences·Elio A SoriaGuillermina A Bongiovanni
May 1, 2016·Physiology & Behavior·Ehsan NassireslamiMohammad Sharifzadeh

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