Cytorhabdovirus P protein suppresses RISC-mediated cleavage and RNA silencing amplification in planta

Virology
Krin S MannRalf G Dietzgen

Abstract

Plant viruses have evolved to undermine the RNA silencing pathway by expressing suppressor protein(s) that interfere with one or more key components of this antiviral defense. Here we show that the recently identified RNA silencing suppressor (RSS) of lettuce necrotic yellows virus (LNYV), phosphoprotein P, binds to RNA silencing machinery proteins AGO1, AGO2, AGO4, RDR6 and SGS3 in protein-protein interaction assays when transiently expressed. In planta, we demonstrate that LNYV P inhibits miRNA-guided AGO1 cleavage and translational repression, and RDR6/SGS3-dependent amplification of silencing. Analysis of LNYV P deletion mutants identified a C-terminal protein domain essential for both local RNA silencing suppression and interaction with AGO1, AGO2, AGO4, RDR6 and SGS3. In contrast to other viral RSS known to disrupt AGO activity, LNYV P sequence does not contain any recognizable GW/WG or F-box motifs. This suggests that LNYV P may represent a new class of AGO binding proteins.

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Citations

Jul 28, 2016·Viruses·Marcio Hedil, Richard Kormelink
Oct 27, 2016·Annals of Botany·Iara P Calil, Elizabeth P B Fontes
Nov 12, 2016·Journal of Virological Methods·Krin S Mann, Ralf G Dietzgen
Dec 25, 2017·Archives of Virology·Mozhgan HortamaniKeramat Izadpanah
Aug 25, 2020·Journal of Experimental Botany·Guilherme Silva-MartinsPeter Moffett
Apr 5, 2018·The Journal of General Virology·Gavin AndersonMichael Goodin
Jul 14, 2020·Frontiers in Microbiology·Mikhail Oliveira LeastroJesús Ángel Sánchez-Navarro

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