Cytoskeletal actin reorganization in neutrophils from patients with localized juvenile periodontitis

Journal of Periodontology
C M ChampagneT E Van Dyke

Abstract

Localized juvenile periodontitis (LJP) is an early-onset periodontal disease associated with a polymorphonuclear neutrophil (PMN) defective migratory response. Kinetics of actin polymerization-depolymerization determine the shape changes occurring in the plasma membrane-associated cytoskeleton and provide the driving force for directed cell migration (chemotaxis). Therefore, we investigated the relation between an abnormality in LJP PMN chemotaxis and an altered reorganization of the actin filament network. PMNs isolated from peripheral blood of LJP patients (n=14) and matching control subjects (n=12) were evaluated for random and directed migration in a Boyden chamber assay, and the kinetics of actin polymerization were studied by flow cytometry. Three groups of LJP patients could be distinguished on the basis of their PMN-chemotactic response compared to their matched control: depressed (n=6), normal (n=4), and elevated (n=4). The abnormal (depressed or elevated) chemotaxis was generally not related to abnormal random migratory response, except for two patients. Since the kinetics of formyl-methionyl-leucyl-phenylalanine-induced F-actin response were highly variable from one subject to another, means were calculated at each t...Continue Reading

References

Feb 1, 1992·The Journal of Cell Biology·G P DowneyS Grinstein
Nov 1, 1991·Biochemical Society Transactions·N Crawford, P Eggleton
Apr 1, 1990·Infection and Immunity·H RepoM Leirisalo-Repo
Mar 1, 1990·Journal of Periodontology·T E Van DykeS Offenbacher
Mar 1, 1990·Infection·B OrenH Hass
Sep 1, 1985·Journal of Periodontal Research·T E Van DykeR J Genco
Sep 10, 1987·The New England Journal of Medicine·H L Malech, J I Gallin
Aug 21, 1981·Science·R Snyderman, E J Goetzl
Jan 1, 1980·Hospital Practice·M Turck
Jan 1, 1980·Infection and Immunity·T E Van DykeR J Genco
Oct 1, 1994·Oral Microbiology and Immunology·S AgarwalM B Aichelmann-Reidy
Mar 1, 1994·Journal of Periodontal Research·S AgarwalA E Riccelli
May 1, 1993·Infection·Y LermanY L Danon
Jul 1, 1993·Journal of Periodontology·M A DanielT E Van Dyke
May 21, 1993·Science·T P Stossel

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Citations

Jan 5, 2000·Journal of Immunological Methods·S F van EedenJ C Hogg
May 30, 2014·Journal of Indian Society of Periodontology·Debabrata KunduMoumita Nayek
Nov 2, 2014·Journal of Clinical Periodontology·Helen M RobertsIain L C Chapple
Jan 14, 2004·Periodontology 2000·Sinem E Sahingur, Robert E Cohen
Aug 31, 2002·Journal of Periodontal Research·Mark I RyderWilliam Hyun

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