PMID: 8587653Aug 1, 1995Paper

Cytoskeletal-associated protein kinase and phosphatase activities from cerebral cortex of young rats

Neurochemical Research
M S de FreitasR Pessoa-Pureur

Abstract

We describe the phosphorylation system associated with the Triton-insoluble cytoskeletal fraction that phosphorylates in vitro the 150 kDa neurofilament subunit (NF-M) and alpha and beta tubulin from cerebral cortex of rats. The protein kinase activities were determined in the presence of 20 microM cyclic AMP (cAMP), 1 mM calcium and 1 microM calmodulin (Ca2+/calmodulin) or 1 mM calcium, 0.2 mM phosphatidylserine and 0.5 microM phorbol 12,13-dibutyrate (Ca2+/PS/PDBu). Phosphorylation of these cytoskeletal proteins increased approximately 35% and 65% in the presence of cAMP and Ca2+/calmodulin, respectively, but was unaffected in the presence of Ca2+/PS/PDBu. Basal phosphorylation of these proteins studied increased approximately 35% and 72% in the presence of 0.5 microM okadaic acid and 0.01 microM microcystin-LR, respectively, suggesting the presence of phosphatase type 1. Results suggest that at least two protein kinases and one protein phosphatase are associated with the Triton-insoluble cytoskeletal fraction from cerebral cortex of rats.

References

Dec 1, 1992·Journal of Neurochemistry·B E Crute, R G Van Buskirk
Jan 1, 1991·Molecular Neurobiology·A T Sim
Jun 29, 1990·Biochemical and Biophysical Research Communications·T TokuiM Inagaki
Feb 1, 1989·The Journal of Cell Biology·P J Hollenbeck
Jan 1, 1989·Journal of Neuropathology and Experimental Neurology·S MonacoP Gambetti
Aug 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·K M YamadaN K Wessells
May 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·N W SeedsM W Nirenberg

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Citations

May 10, 2003·Brain Research. Brain Research Protocols·Cláudia FunchalRegina Pessoa Pureur
May 1, 1996·Neurochemical Research·M Camargo-De-MoraisC A Netto

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