Cytoskeletal changes and the system of regulation of alkaline phosphatase activity in human periodontal ligament cells induced by mechanical stress

Cell Biochemistry and Function
Mirei Chiba, Hideo Mitani

Abstract

The periodontal ligament (PDL) is a specialized, mechanically responsive tissue that adapts via cellular responses to equilibrate the effects of mechanical stress on teeth. However, the mechanism of remodelling by which individual cells in periodontal tissue detect and respond to mechanical stress is not well understood. To identify the cellular mechanisms induced by mechanical stress in the periodontal ligament, we examined the effects of cyclic stretching on periodontal ligament fibroblast-like cells (PDL cells). Furthermore, we investigated the effects of 1alpha,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3)), and interaction with peripheral blood mononuclear cells (PBMCs) on mechanically-simulated PDL cells. PDL cells were cultured on type I collagen-coated silicon membranes with 10% FBS alpha-MEM, and then subjected to cyclic mechanical stimulation (1 s stretching/1 s relaxation, 15% maximum elongation). Alkaline phosphatase activity was monitored by cytochemical and spectrophotometric methods. Morphologically, the cells assumed a spindle shape, and the cytoskeletal components, including microtubules and F-actin filaments, were aligned perpendicular to the strain force vector. Cyclic stretching decreased ALPase activity in PDL ...Continue Reading

Citations

Dec 21, 2012·Journal of Investigative and Clinical Dentistry·Shabnam Soltani BozchaloeiMahvash Oskoui
Jul 23, 2011·Journal of Periodontal Research·C XuF Zhang
Nov 19, 2011·Journal of Clinical Periodontology·Sahng G KimHyun-Duck Nah
Nov 27, 2007·Journal of Dental Research·D C WescottM C Meikle
Oct 7, 2010·Journal of Dental Research·Y-G KangK-S Lee
Feb 28, 2018·International Journal of Oral Science·Chia-Ying YangDana T Graves
Jan 23, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall
Dec 10, 2016·European Journal of Orthodontics·Adamantia PapadopoulouDimitris Kletsas
Oct 18, 2020·Journal of Biomechanical Engineering·R GauthierB Grosgogeat
Mar 16, 2016·Journal of Tissue Engineering·Hye-Sun YuIvan Wall
Apr 13, 2021·ACS Biomaterials Science & Engineering·Sara Correia CarreiraJonathan Rossiter

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