PMID: 19920666Jun 1, 1995Paper

Cytoskeletal changes in cultured human glaucoma trabecular meshwork cells

Journal of Glaucoma
A F ClarkM D McCartney

Abstract

Glucocorticoid treatment of cultured human trabecular meshwork (TM) cells has been shown to reorganize actin microfilaments into cross-linked actin networks (CLANs) and to alter TM cell function. The purpose of the present study is to determine whether similar microfilament structural changes occur in TM cells derived from glaucoma patients. The microfilament structures of nine different TM cell cultures, derived from four glaucoma patients and from five normal subjects, was examined by epifluorescent microscopy and by whole-mount transmission electron microscopy. The cells were cultured in the absence and presence of dexamethasone (DEX) for 7-14 days. The normal TM cell lines and glaucoma cell line TM13 had relatively low levels of cross-linked actin networks in the absence of exogenously added glucocorticoids. In contrast, the glaucoma cell lines TM23, TM36C, and TM48D had high levels of CLANs without the addition of dexamethasone. The addition of 10 M DEX to the culture medium significantly increased CLAN expression in all normal as well as glaucoma TM cell lines. CLAN formation in glaucoma TM cells or in TM cells exposed to glucocorticoids may be a factor contributing to the generation of ocular hypertension.

Citations

Nov 22, 2011·Cell and Tissue Research·Rudolf Fuchshofer, Ernst R Tamm
Aug 7, 1999·Progress in Retinal and Eye Research·R J Wordinger, A F Clark
Jun 23, 2009·Experimental Eye Research·N C WadeA F Clark
Nov 4, 2008·Experimental Eye Research·Abbot F Clark, Robert J Wordinger
Jan 16, 2007·Experimental Eye Research·A Thomas ReadC Ross Ethier
Jun 9, 2015·Experimental Eye Research·Darryl R Overby, Abbot F Clark
Aug 19, 2015·Biochimica Et Biophysica Acta·Ling YuDouglas C Eaton
Dec 6, 2005·Experimental Eye Research·A Thomas ReadC Ross Ethier
Dec 2, 2005·Experimental Eye Research·Mitchell D McCartneyAbbot F Clark
Oct 9, 2013·Biochimica Et Biophysica Acta·Jennifer A FaralliDonna M Peters
Apr 20, 2016·Experimental Eye Research·Hannah C WebberWeiming Mao
Jun 19, 2013·Progress in Retinal and Eye Research·Toshihiro Inoue, Hidenobu Tanihara
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
May 18, 2016·Experimental Eye Research·Mark S FillaDonna M Peters
Jul 23, 2016·Experimental Eye Research·W Daniel Stamer, Abbot F Clark
Sep 27, 2016·Progress in Retinal and Eye Research·M Elizabeth FiniW Daniel Stamer

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