Cytoskeletal components of an invasion machine--the apical complex of Toxoplasma gondii

PLoS Pathogens
Ke HuJohn M Murray

Abstract

The apical complex of Toxoplasma gondii is widely believed to serve essential functions in both invasion of its host cells (including human cells), and in replication of the parasite. The understanding of apical complex function, the basis for its novel structure, and the mechanism for its motility are greatly impeded by lack of knowledge of its molecular composition. We have partially purified the conoid/apical complex, identified approximately 200 proteins that represent 70% of its cytoskeletal protein components, characterized seven novel proteins, and determined the sequence of recruitment of five of these proteins into the cytoskeleton during cell division. Our results provide new markers for the different subcompartments within the apical complex, and revealed previously unknown cellular compartments, which facilitate our understanding of how the invasion machinery is built. Surprisingly, the extreme apical and extreme basal structures of this highly polarized cell originate in the same location and at the same time very early during parasite replication.

References

Aug 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D LuckB Huang
Dec 27, 1977·Zeitschrift Für Parasitenkunde·E Porchet, G Torpier
Feb 1, 1978·Journal of Ultrastructure Research·J F Dubremetz, G Torpier
Jan 1, 1988·Parasitology Research·T EndoT Nakamura
Nov 1, 1988·The Journal of protozoology·N D Levine
Jan 1, 1988·Parasitology Research·T YasudaT Endo
Jun 1, 1988·Molecular and Biochemical Parasitology·S D Nagel, J C Boothroyd
May 1, 1987·The Journal of protozoology·B A Nichols, M L Chiappino
Mar 1, 1985·The Journal of Cell Biology·G Sluder, C L Rieder
Feb 1, 1968·The Journal of Cell Biology·E RobbinsA Micali
Nov 1, 1983·The Journal of protozoology·J D Schwartzman, E R Pfefferkorn
Feb 1, 1995·Current Opinion in Cell Biology·J L Salisbury
Jul 29, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R K Colwell, J A Coddington
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Donald, D S Roos
Mar 1, 1996·The Journal of Eukaryotic Microbiology·R Mondragon, E Frixione
Dec 1, 1996·Experimental Parasitology·T J StokkermansD S Roos
Jul 24, 1998·Experimental Parasitology·N S Morrissette, D S Roos
Sep 10, 1998·The Journal of Cell Biology·E BiJ R Pringle
May 26, 1999·Molecular and Biochemical Parasitology·F SeeberH H Schmidt
Sep 2, 1999·Cell Motility and the Cytoskeleton·M B Heintzelman, J D Schwartzman
Aug 19, 2000·Molecular Cell·H TakeshimaK Kangawa
Jan 3, 2001·The Journal of Cell Biology·B StriepenD S Roos
Mar 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N Pezzella-D'AlessandroJ M Pinon
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jason R SwedlowJohn M Murray
Feb 21, 2002·Molecular Biology of the Cell·Ke HuJohn M Murray
Mar 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Naomi S Morrissette, L David Sibley
Mar 20, 2002·The Journal of Cell Biology·Ke HuJohn M Murray
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Jul 4, 2002·Molecular & Cellular Proteomics : MCP·Aaron J MackeyWilliam R Pearson
Jul 30, 2002·Omics : a Journal of Integrative Biology·Andrew KellerEugene Kolker
Aug 15, 2002·The Journal of Biological Chemistry·Tara MannCon Beckers
Oct 9, 2002·Nature·Laurence FlorensDaniel J Carucci
Mar 13, 2003·Molecular Biology of the Cell·Michelle Piehl, Lynne Cassimeris
Apr 15, 2003·Nature Biotechnology·Christine C WuJohn R Yates
Apr 13, 2004·Molecular & Cellular Proteomics : MCP·Steven CarrUNKNOWN Working Group on Publication Guidelines for Peptide and Protein Identification Data
Oct 21, 2004·Journal of Cell Science·Ke HuJohn M Murray
Jul 9, 2005·The Journal of Biological Chemistry·Xing W ZhouVern B Carruthers
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates

❮ Previous
Next ❯

Citations

Oct 25, 2012·Journal of Biomedicine & Biotechnology·Maria Martha CorviMarina Cecilia Caballero
Feb 19, 2014·Parasitology·David M Witcombe, Nicholas C Smith
Jul 3, 2007·PLoS Pathogens·Boris StriepenGiel G van Dooren
Nov 16, 2007·Nucleic Acids Research·Bindu GajriaBrian P Brunk
Feb 20, 2008·PLoS Pathogens·Marc-Jan GubbelsMichael W White
Mar 4, 2008·Eukaryotic Cell·David J P FergusonMarc-Jan Gubbels
Feb 17, 2009·Eukaryotic Cell·My-Hang Huynh, Vern B Carruthers
Feb 9, 2010·PLoS Pathogens·Aoife T HeaslipKe Hu
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Carrie F BrooksBoris Striepen
Aug 9, 2011·BMC Bioinformatics·Sabina LeonelliJane Lomax
Sep 2, 2011·BMC Biology·Wallace F Marshall
Oct 25, 2011·Eukaryotic Cell·Johnson Q TranNaomi S Morrissette
Jul 29, 2009·Biomolecular NMR Assignments·Bing LiuStephen Matthews
Mar 25, 2008·Molecular Biology and Evolution·Sven B GouldRoss F Waller
Feb 10, 2016·International Journal for Parasitology·S SkariahD G Mordue
Mar 26, 2014·Journal of Proteomics·Carmen T Gómez de LeónRicardo Mondragón Flores
Sep 18, 2012·Molecular and Biochemical Parasitology·Ben CowperFiona Tomley
Jun 11, 2011·Trends in Parasitology·Jessica S TylerJohn C Boothroyd
Apr 6, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ferenc Orosz
Sep 26, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ferenc Orosz
Jan 17, 2009·Parasitology International·William D Nevin, Joel B Dacks
Aug 2, 2008·Microbes and Infection·Kami Kim, Louis M Weiss
Jun 21, 2008·International Journal of Pharmaceutics·M J PrietoE L Romero
May 19, 2009·Molecular and Biochemical Parasitology·Brooke MorriswoodGraham Warren
May 7, 2009·Cellular Microbiology·M González Del CarmenR Mondragón
Nov 30, 2007·Journal of Microscopy·John M MurrayJennifer C Waters
Sep 4, 2015·Annual Review of Microbiology·Ira J BladerMarc-Jan Gubbels
Jan 17, 2007·Cellular Microbiology·Cynthia Y He
Sep 14, 2006·Molecular Microbiology·Shipra Vaishnava, Boris Striepen

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
protein assay
Fluorescence
electron microscopy
light microscopy
transgenics
PCR

Software Mentioned

SEQUEST
BLAST
EstimateS
MudPIT
FASTS34
DTASelect
FASTA
Fortran
PROBE
BLASTALL

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.