Cytoskeletal pinning controls phase separation in multicomponent lipid membranes

Biophysical Journal
Senthil ArumugamPetra Schwille

Abstract

We study the effect of a minimal cytoskeletal network formed on the surface of giant unilamellar vesicles by the prokaryotic tubulin homolog, FtsZ, on phase separation in freestanding lipid membranes. FtsZ has been modified to interact with the membrane through a membrane targeting sequence from the prokaryotic protein MinD. FtsZ with the attached membrane targeting sequence efficiently forms a highly interconnected network on membranes with a concentration-dependent mesh size, much similar to the eukaryotic cytoskeletal network underlying the plasma membrane. Using giant unilamellar vesicles formed from a quaternary lipid mixture, we demonstrate that the artificial membrane-associated cytoskeleton, on the one hand, suppresses large-scale phase separation below the phase transition temperature, and, on the other hand, preserves phase separation above the transition temperature. Our experimental observations support the ideas put forward in our previous simulation study: In particular, the picket fence effect on phase separation may explain why micrometer-scale membrane domains are observed in isolated, cytoskeleton-free giant plasma membrane vesicles, but not in intact cell membranes. The experimentally observed suppression of ...Continue Reading

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Citations

Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Darius Vasco KösterSatyajit Mayor
Mar 21, 2016·Biochimica Et Biophysica Acta·Tripta BhatiaOle G Mouritsen
Apr 4, 2016·Biochimica Et Biophysica Acta·Christian Eggeling, Alf Honigmann
Oct 22, 2016·Journal of Molecular Biology·Alf Honigmann, Arnd Pralle
Nov 2, 2017·Nature Communications·Joseph H LorentIlya Levental
Jan 4, 2019·Acta Crystallographica. Section D, Structural Biology·Michael Weinrich, David L Worcester
Aug 1, 2020·Angewandte Chemie·Kristina A GanzingerPetra Schwille
Apr 30, 2020·Chembiochem : a European Journal of Chemical Biology·Geralin A HögerUlf Diederichsen
Dec 30, 2018·Proceedings of the National Academy of Sciences of the United States of America·Nick Vallespir Lowery, Tristan Ursell
Jul 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ole M SchütteClaudia Steinem
Jan 26, 2017·Frontiers in Cell and Developmental Biology·Toyoshi Fujimoto, Ingela Parmryd
Nov 2, 2019·Archives of Biochemistry and Biophysics·Mohammad A A FakhreeMireille M A E Claessens
Mar 30, 2021·Applied Magnetic Resonance·N Stein, W K Subczynski
Jun 4, 2021·Journal of Chemical Information and Modeling·Maria C OliveiraRodrigo M Cordeiro
Jun 18, 2021·Nature Communications·Senthil ArumugamLudger Johannes
Jul 23, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Semen V NesterovVladimir N Uversky
Oct 19, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·James A GoodchildSimon D Connell
Mar 7, 2018·Biochemistry·Alan K ItakuraDaniel F Jarosz
May 22, 2020·The Journal of Physical Chemistry. B·Ivan Castello-SerranoIlya Levental

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