PMID: 9168148Apr 26, 1997Paper

Cytoskeleton-membrane connections in the human erythrocyte membrane: band 4.1 binds to tetrameric band 3 protein

Biochimica Et Biophysica Acta
B von RückmannD Schubert

Abstract

Band 4.1 provides, besides ankyrin, the main linkage between the erythrocyte membrane and its cytoskeleton. Its predominant binding sites in the membrane are located on the glycophorins. However, the cytoplasmic domain of band 3 can also bind band 4.1. We have studied which of the different band 3 oligomers observed (monomers, dimers, tetramers) can act as band 4.1 binding sites, by equilibrium sedimentation experiments on mixtures of purified band 3 and dye-labelled band 4.1 in solutions of a nonionic detergent. At low molar ratios of band 4.1 and band 3, the sedimentation equilibrium distributions obtained could all be perfectly fitted assuming that only two dye-labelled particles were present: uncomplexed band 4.1 and a complex formed between one band 4.1 molecule and one band 3 tetramer. The presence of small amounts of complexes containing band 3 monomers or dimers could not be completely ruled out but is unlikely. On the other hand, stabilized band 3 dimers effectively bound band 4.1. At higher molar band 4.1/band 3 ratio, the band 3 tetramer apparently could bind up to at least four band 4.1 molecules. The band 4.1/band 3 tetramer complex was found to be unstable. The results described, together with those reported previ...Continue Reading

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S Lindenthal, D Schubert
Apr 24, 1984·FEBS Letters·J C PinderW B Gratzer

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Citations

May 23, 2001·Progress in Neurobiology·N C Danbolt
Mar 13, 2003·Kidney International·Mohamed RamezPhilippe Gascard
Mar 8, 2000·Biochimica Et Biophysica Acta·M M Cruz SilvaJ B Custódio
Mar 20, 2014·Free Radical Biology & Medicine·Mattia ZaccarinStefano Toppo
Nov 30, 2010·Journal of Biomechanics·Jamie L Maciaszek, George Lykotrafitis
Aug 5, 2003·Journal of Cellular Biochemistry·Paulo Roberto Moura LimaSara Teresinha Olalla Saad
Jan 15, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Paola MatarreseWalter Malorni
Jun 18, 2005·Anatomical Science International·Nobuo TeradaShinichi Ohno

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