Cytosolic accumulation of small nucleolar RNAs (snoRNAs) is dynamically regulated by NADPH oxidase.

The Journal of Biological Chemistry
Christopher L HolleyJean E Schaffer

Abstract

Small nucleolar RNAs (snoRNAs) guide nucleotide modifications of cellular RNAs in the nucleus. We previously showed that box C/D snoRNAs from the Rpl13a locus are unexpected mediators of physiologic oxidative stress, independent of their predicted ribosomal RNA modifications. Here we demonstrate that oxidative stress induced by doxorubicin causes rapid cytoplasmic accumulation of the Rpl13a snoRNAs through a mechanism that requires superoxide and a nuclear splice variant of NADPH oxidase. RNA-sequencing analysis reveals that box C/D snoRNAs as a class are present in the cytoplasm, where their levels are dynamically regulated by NADPH oxidase. These findings suggest that snoRNAs may orchestrate the response to environmental stress through molecular interactions outside of the nucleus.

References

Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
Jun 9, 2005·The Journal of Cell Biology·Patrice VitaliAlexander Huttenhofer
Dec 17, 2005·Science·Shivendra Kishore, Stefan Stamm
Feb 6, 2007·Free Radical Biology & Medicine·Shiwei DengLeszek Wojnowski
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·A Gregory MateraMichael P Terns
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·T KissM Weber
Aug 22, 2007·Molecular and Cellular Biology·Nicholas J WatkinsReinhard Lührmann
Dec 17, 2009·BMC Research Notes·Paul Holden, William A Horton
Jun 3, 2010·Cold Spring Harbor Protocols·Donald C RioTimothy W Nilsen
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Daniele AvitabileMark A Sussman
Jul 5, 2011·Cell Metabolism·Carlos I MichelJean E Schaffer
Aug 8, 2012·Molecular and Cellular Biology·Benjamin S ScruggsJean E Schaffer
Oct 2, 2012·Molecular Cell·Thomas SchubertGernot Längst
Feb 9, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Narayana AnilkumarAjay M Shah

❮ Previous
Next ❯

Citations

Jul 25, 2015·Cell Death and Differentiation·G CaputaJ E Schaffer
Jun 12, 2016·Diabetes·George Caputa, Jean E Schaffer
Nov 8, 2016·The Journal of Clinical Investigation·Jiyeon LeeJean E Schaffer
May 16, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Marina FalaleevaStefan Stamm
May 5, 2017·Annals of Neurology·Qiao-Ling CuiJack P Antel
Mar 2, 2018·Genes·Alexandra Iulia IrimieIoana Berindan-Neagoe
Aug 18, 2017·Antioxidants & Redox Signaling·Matthias S LeisegangRalf P Brandes
Oct 13, 2017·The Journal of Biological Chemistry·Melissa W LiJean E Schaffer
Jul 8, 2018·The Journal of Biological Chemistry·Jamie M RimerJean E Schaffer
Apr 11, 2019·Physiology·Philip D Stahl, Graca Raposo
Dec 13, 2019·Nucleic Acids Research·Tomaž BratkovičBoris Rogelj
May 13, 2020·The Journal of Biological Chemistry·Jean E Schaffer
Aug 23, 2020·International Journal of Molecular Sciences·Alina-Andreea ZimtaCiprian Tomuleasa
Aug 1, 2019·Nature Communications·Brittany A ElliottChristopher L Holley
Nov 30, 2019·Frontiers in Pharmacology·Julia A FilippovaGrigory A Stepanov
Aug 20, 2020·Circulation. Genomic and Precision Medicine·Saumya DasUNKNOWN American Heart Association Council on Genomic and Precision Medicine; Council on Arteriosclerosis, Thrombosis and Vascular B
Jul 1, 2020·International Journal of Molecular Sciences·Martina GodelJoanna Kopecka
Jan 6, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Venzil Lavie DsouzaShama Prasada Kabekkodu
Dec 7, 2020·Journal of Molecular and Cellular Cardiology·K Shanmugha RajanVemparthan Suvekbala
Jun 6, 2021·Genome Biology·Étienne Fafard-CoutureMichelle S Scott
Nov 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Gaëlle J S TalrossJoseph G Gall

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.