Cytosolic APx knockdown indicates an ambiguous redox responses in rice

Phytochemistry
Silvia Barcelos RosaMárcia Margis-Pinheiro

Abstract

Ascorbate peroxidases (APX, EC 1.1.11.1) are class I heme-peroxidases, which catalyze the conversion of H(2)O(2) into H(2)O, using ascorbate as a specific electron donor. Previously, the presence of eight Apx genes was identified in the nuclear genome of rice (Oryza sativa), encoding isoforms that are located in different sub-cellular compartments. Herein, the generation of rice transgenic plants silenced for either both or each one of the cytosolic Apx1 and Apx2 genes was carried out in order to investigate the importance of cytosolic Apx isoforms on plant development and on plant stress responses. Transgenic double Apx1/2-silenced plants exhibited normal development, even though these plants showed a global reduction of Apx activity which strongly impacts the whole antioxidant system regulation. Apx1/2-silenced plants also showed increased H(2)O(2) accumulation under control and stress situations and presented higher tolerance to toxic concentration of aluminum when compared to wild type plants. On the other hand, silencing OsApx1 and OsApx2 genes individually resulted in strong effect on plant development producing semi-dwarf phenotype. These results suggested that the double silencing of cytosolic OsApx genes induced compen...Continue Reading

References

Feb 10, 1995·Analytical Biochemistry·K KampfenkelD Inzé
Sep 2, 1999·Analytical Biochemistry·C GayJ M Gebicki
Jun 6, 2000·Current Opinion in Plant Biology·N Smirnoff
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 9, 2002·Journal of Experimental Botany·Shigeru ShigeokaKazuya Yoshimura
Dec 21, 2002·The Plant Journal : for Cell and Molecular Biology·Yukinori YabutaShigeru Shigeoka
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Kozi Asada
Apr 28, 2004·Plant & Cell Physiology·Daisuke Miki, Ko Shimamoto
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
Dec 16, 2004·Journal of Molecular Evolution·Felipe Karam TeixeiraMárcia Margis-Pinheiro
Jun 9, 2006·Plant Physiology·Philip M MullineauxNeil R Baker
Jun 13, 2006·Journal of Experimental Botany·John M Cheeseman
Aug 10, 2006·Plant Molecular Biology·Roman A VolkovFriedrich Schöffl
Oct 6, 2007·Journal of Experimental Botany·Chwan-Yang HongChing Huei Kao
Jun 6, 2008·Plant Physiology·Andrew EamensPeter M Waterhouse

❮ Previous
Next ❯

Citations

Nov 26, 2013·Plant Science : an International Journal of Experimental Plant Biology·Andréia CaverzanMárcia Margis-Pinheiro
Mar 22, 2012·Omics : a Journal of Integrative Biology·F Vanessa Loiacono, Mario C De Tullio
May 21, 2013·Plant Science : an International Journal of Experimental Plant Biology·Gisele PassaiaMarcia Margis-Pinheiro
Jun 9, 2012·Plant, Cell & Environment·Rafael Augusto ArenhartMárcia Margis-Pinheiro
Sep 12, 2014·Bioscience, Biotechnology, and Biochemistry·Shigeru Shigeoka, Takanori Maruta
Aug 24, 2016·Environmental Science and Pollution Research International·Naser A AnjumNarendra Tuteja
Mar 24, 2016·Genetics and Molecular Biology·Andréia CaverzanSandra Patussi Brammer
Feb 16, 2013·Genetics and Molecular Biology·Andréia CaverzanMárcia Margis-Pinheiro
Oct 1, 2011·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Renu Khanna-Chopra, Vimal Kumar Semwal
Dec 19, 2019·Antioxidants·Karolina BilskaEwa Marzena Kalemba
May 6, 2017·Frontiers in Plant Science·Saurabh PandeyMalireddy K Reddy
Dec 1, 2014·Botanical Studies·Jelli Venkatesh, Se Won Park
Jul 14, 2017·Planta·Priscila Ariane AulerEugenia Jacira Bolacel Braga
Jul 28, 2020·International Journal of Molecular Sciences·Damian GruszkaAnna Janeczko
Jan 25, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Sandra Ndagire KamenyaDamaris Achieng Odeny
Mar 7, 2021·International Journal of Molecular Sciences·Kimberly S PonceGuoyou Ye
Mar 24, 2021·International Journal of Biological Macromolecules·Baomei WuWeizhong Liu
Apr 12, 2021·Nitric Oxide : Biology and Chemistry·Ming-Yue WeiHai-Lei Zheng

❮ Previous
Next ❯