Cytosolic lipid droplets as engineered organelles for production and accumulation of terpenoid biomaterials in leaves

Nature Communications
Radin SadreBjoern Hamberger

Abstract

Cytosolic lipid droplets are endoplasmic reticulum-derived organelles typically found in seeds as reservoirs for physiological energy and carbon to fuel germination. Here, we report synthetic biology approaches to co-produce high-value sesqui- or diterpenoids together with lipid droplets in plant leaves. The formation of cytosolic lipid droplets is enhanced in the transient Nicotiana benthamiana system through ectopic production of WRINKLED1, a key regulator of plastid fatty acid biosynthesis, and a microalgal lipid droplet surface protein. Engineering of the pathways providing the universal C5-building blocks for terpenoids and installation of terpenoid biosynthetic pathways through direction of the enzymes to native and non-native compartments boost the production of target terpenoids. We show that anchoring of distinct biosynthetic steps onto the surface of lipid droplets leads to efficient production of terpenoid scaffolds and functionalized terpenoids. The co-produced lipid droplets "trap" the terpenoids in the cells.

References

Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·O VoinnetD C Baulcombe
Jul 19, 2002·The Journal of Biological Chemistry·Ming LiJao-Yiu Sung
Oct 27, 2004·The Plant Journal : for Cell and Molecular Biology·Alex Cernac, Christoph Benning
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Dec 15, 2010·PloS One·Teun W J M van HerpenJules Beekwilder
Nov 18, 2011·The Journal of Biological Chemistry·Kent D Chapman, John B Ohlrogge
Dec 12, 2012·Nature Protocols·Yunfeng DingPingsheng Liu
Dec 18, 2013·Plant Methods·Kathleen Brückner, Alain Tissier
Apr 9, 2014·Current Opinion in Plant Biology·Hugues RenaultDanièle Werck-Reichhart
Jun 13, 2014·Natural Product Reports·A Banerjee, T D Sharkey
Jan 27, 2015·Annual Review of Plant Biology·Bernd Markus Lange
Jun 11, 2015·Plant Biotechnology Journal·Janice ZaleFredy Altpeter
Aug 1, 2015·Planta·Jörg M AugustinToni M Kutchan
Aug 27, 2015·Proceedings of the National Academy of Sciences of the United States of America
Sep 12, 2015·The New Phytologist·Lemeng DongAlexander Van Der Krol
Mar 21, 2016·Biotechnology Advances·Pan LiaoMee-Len Chye
Mar 31, 2016·Sub-cellular Biochemistry·Zhi-Yan Du, Christoph Benning
Dec 21, 2016·Chemical Reviews·Annika Frank, Michael Groll
Feb 15, 2018·ACS Synthetic Biology·Cheng ZhaoJoshua S Yuan
Apr 24, 2018·Plant Biotechnology Journal·Thierry L DelatteJules Beekwilder

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Citations

Aug 11, 2020·The Plant Journal : for Cell and Molecular Biology·Garret P MillerBjörn Hamberger
Feb 27, 2020·Biology·Ben LeylandInna Khozin-Goldberg
Jul 14, 2020·Journal of Cellular Physiology·Anjana K BadekilaAmit K Jaiswal
Sep 29, 2019·The Journal of Biological Chemistry·Hiroshi A Maeda
Apr 23, 2020·Biotechnology for Biofuels·Chien-Yuan Lin, Aymerick Eudes
Jul 10, 2020·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kin H LauC Robin Buell
Dec 8, 2020·PloS One·Anantha PeramunaHenrik Toft Simonsen
Nov 27, 2020·Frontiers in Chemistry·Kaouthar Eljounaidi, Benjamin R Lichman
Mar 11, 2020·Current Opinion in Biotechnology·Michael S BelcherJay D Keasling
Apr 30, 2020·Progress in Lipid Research·Peter K LundquistRoberto Espinoza-Corral
Oct 12, 2021·The Plant Cell·Byung-Ho KangBethany Karlin Zolman

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Datasets Mentioned

BETA
AY508730

Methods Mentioned

BETA
transgenic

Software Mentioned

FV10
MassLynx
QuanLynx
Graphpad Prism
SnapGene
ASW

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