Cytosolic PLA2(alpha) activation in Purkinje neurons and its role in AMPA-receptor trafficking

Journal of Cell Science
Masato MashimoTakao Shimizu

Abstract

Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) selectively releases arachidonic acid from membrane phospholipids and has been proposed to be involved in the induction of long-term depression (LTD), a form of synaptic plasticity in the cerebellum. This enzyme requires two events for its full activation: Ca(2+)-dependent translocation from the cytosol to organelle membranes in order to access phospholipids as substrates, and phosphorylation by several kinases. However, the subcellular distribution and activation of cPLA(2)alpha in Purkinje cells and the role of arachidonic acid in cerebellar LTD have not been fully elucidated. In cultured Purkinje cells, stimulation of AMPA receptors, but not metabotropic glutamate receptors, triggered translocation of cPLA(2)alpha to the somatic and dendritic Golgi compartments. This translocation required Ca(2+) influx through P-type Ca(2+) channels. AMPA plus PMA, a chemical method for inducing LTD, released arachidonic acid via phosphorylation of cPLA(2)alpha. AMPA plus PMA induced a decrease in surface GluR2 for more than 2 hours. Interestingly, this reduction was occluded by a cPLA(2)alpha-specific inhibitor. Furthermore, PMA plus arachidonic acid caused the prolonged internalization of G...Continue Reading

References

Apr 18, 1994·Brain Research·Y KovalchukD Attwell
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L S MayorgaP D Stahl
Oct 1, 1995·Journal of Lipid Mediators and Cell Signalling·J D ClarkL L Lin
Oct 1, 1996·The European Journal of Neuroscience·A R Boxall, J Garthwaite
Jul 4, 1997·The Journal of Biological Chemistry·C C Leslie
Jan 15, 1999·Science·U S Bhalla, R Iyengar
May 1, 1999·The Journal of Biological Chemistry·H KawasakiT Hirano
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·M U EhrengruberB H Gähwiler
Nov 18, 2000·Biochimica Et Biophysica Acta·T Hirabayashi, T Shimizu
Nov 25, 2000·Cellular and Molecular Life Sciences : CMLS·G Massicotte
Feb 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H SanoA R Leff
May 1, 2001·The Journal of Biological Chemistry·J T O'FlahertyL W Daniel
May 26, 2001·The Journal of Biological Chemistry·J H EvansC C Leslie
Nov 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D J Linden
Dec 6, 2001·Nature Reviews. Neuroscience·C Job, J Eberwine
Jan 24, 2002·Molecular Cell·Diana Murray, Barry Honig
Jul 30, 2003·The Journal of Biological Chemistry·Sudipto DasWonhwa Cho
Dec 3, 2003·American Journal of Physiology. Renal Physiology·Andrey V CybulskyLudmilla Le Berre
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Christina C Leslie
Aug 12, 2004·Biological & Pharmaceutical Bulletin·Ichiro Kudo
Aug 12, 2004·Biological & Pharmaceutical Bulletin·Tetsuya HirabayashiTakao Shimizu
Oct 12, 2004·Journal of Neurocytology·Yoshinori Shirai, Masao Ito
Nov 19, 2004·The EMBO Journal·Stéphane Martin, Jeremy M Henley

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.