PMID: 8614623Apr 1, 1996Paper

Cytosolic yeast tRNA(His) is covalently modified when imported into mitochondria of Trypanosoma brucei

Nucleic Acids Research
A Schneider

Abstract

The mitochondrial genome of Trypanosoma brucei does not encode any tRNAs. Instead, mitochondrial tRNAs are synthesized in the nucleus and subsequently imported into mitochondria. The great majority of mitochondrial tRNAs have cytosolic counterparts showing identical primary sequences. The only difference found between mitochondrial and cytosolic isotypes of the tRNAs are mitochondria-specific nucleotide modifications which appear to be a common feature of imported tRNAs in trypanosomes. In this study, a mutated yeast cytosolic tRNAHis was expressed in trypanosomes and its import phenotype was analyzed by cell fractionation and nuclease treatment of intact mitochondria. Furthermore, cytosolic and mitochondrial isotypes of the yeast tRNA(His) were specifically labeled and analyzed by limited alkaline hydrolysis. These experiments revealed the presence of mitochondria-specific nucleotide modifications in the yeast tRNA(His). The positions of the modifications were determined by direct enzymatic sequencing of the tRNA(His) and shown to correspond to the ultimate and penultimate nucleotides before the anticodon, the same relative positions which are modified in the mitochondrial isotype of trypanosomal tRNA(Tyr). The results demonst...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·A DietrichL Maréchal-Drouard
Sep 9, 1988·Science·K Verner, G Schatz
Jun 10, 1988·Nucleic Acids Research·L Maréchal-DrouardP Guillemaut
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Metzenberg, N Agabian
Apr 1, 1994·Molecular and Cellular Biology·A SchneiderN Agabian
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·A HehlI Roditi
Jan 1, 1996·Nucleic Acids Research·M SprinzlS Steinberg
Aug 1, 1994·Trends in Cell Biology·A Schneider

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