Cytostatic and genotoxic effect of temephos in human lymphocytes and HepG2 cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
A B Benitez-TrinidadA E Rojas-García

Abstract

Temephos is an organophosphorus pesticide that is used in control campaigns against Aedes aegypti mosquitoes, which transmit dengue. In spite of the widespread use of temephos, few studies have examined its genotoxic potential. The aim of this study was to evaluate the cytotoxic, cytostatic and genotoxic effects of temephos in human lymphocytes and hepatoma cells (HepG2). The cytotoxicity was evaluated with simultaneous staining (FDA/EtBr). The cytostatic and genotoxic effects were evaluated using comet assays and the micronucleus technique. We found that temephos was not cytotoxic in either lymphocytes or HepG2 cells. Regarding the cytostatic effect in human lymphocytes, temephos (10 μM) caused a significant decrease in the percentage of binucleated cells and in the nuclear division index as well as an increase in the apoptotic cell frequency, which was not the case for HepG2 cells. The comet assay showed that temephos increased the DNA damage levels in human lymphocytes, but it did not increase the MN frequency. In contrast, in HepG2 cells, temephos increased the tail length, tail moment and MN frequency in HepG2 cells compared to control cells. In conclusion, temephos causes stable DNA damage in HepG2 cells but not in human ...Continue Reading

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Citations

Sep 27, 2016·Environmental Pollution·Viera SchwarzbacherováJán Dianovský
Jul 19, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xiaoran DuanWei Wang
Jan 31, 2018·International Journal of Environmental Health Research·G A Toledo-IbarraM I Girón-Pérez
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Aug 14, 2020·Journal of Toxicology and Environmental Health. Part a·Daniel Luís Viana CruzMíriam Silva Rafael
Sep 13, 2018·Environmental Science and Pollution Research International·Maria Del Carmen Xotlanihua-GervacioAurora Elizabeth Rojas-García
Mar 30, 2019·Environmental Science and Pollution Research International·José Francisco Herrera-MorenoAurora Elizabeth Rojas-García
Jan 21, 2021·Archives of Toxicology·Francisco Alberto Verdín-BetancourtAdolfo Sierra-Santoyo
Jul 3, 2021·Toxics·Luiza Flavia Veiga FranciscoAlexeia Barufatti
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Aug 13, 2021·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ángel Ramos-FloresBetzabet Quintanilla-Vega

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