Cytotoxic activity generated from channel catfish peripheral blood leukocytes in mixed leukocyte cultures

Cellular Immunology
T StugeL W Clem

Abstract

In previous work, lysis of allotargets was routinely observed with PBL from nonimmune channel catfish. In the work reported here, greatly increased (approximately 100-fold) cytotoxic responses were generated by stimulation of channel catfish PBL with irradiated cells of allogeneic cloned B cell lines in mixed leukocyte cultures (MLC). This increased cytotoxicity did not appear to be simply a consequence of cell proliferation since stimulation of catfish PBL proliferative responses with polyclonal mitogens did not result in increased lysis. Somewhat surprisingly, the MLC-generated cytotoxicity did not exhibit allospecificity; i.e., allogeneic targets from other fish were as susceptible to lysis as were the cells used as stimulators. This apparent lack of allospecificity in MLC-generated cytotoxicity was confirmed by "cold" target inhibition assays. However, autologous targets were not killed, clearly demonstrating that MLC-generated effectors could distinguish "self" from "nonself" at the level of lysis/recognition. Although their origin is unresolved, the MLC-generated effectors may be a source of highly enriched fish cytotoxic cells and thus facilitate directly addressing questions pertaining to the evolution of such cells.

References

Jun 1, 2000·Developmental and Comparative Immunology·K S BarkerN W Miller

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Citations

Jan 23, 1999·Immunological Reviews·N MillerW Clem
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A HawkeG W Litman
Apr 29, 1999·Developmental and Comparative Immunology·L Jaso-Friedmann, D L Evans
Oct 16, 2002·Developmental and Comparative Immunology·H ParkN W Miller
Feb 10, 2006·Immunogenetics·Ivanka Milev-MilovanovicV G Chinchar
Apr 14, 2012·Analytical Biochemistry·Marie-Aline PierrardFrédéric Silvestre
May 3, 2011·Developmental and Comparative Immunology·Teruyuki NakanishiTomonori Somamoto
Apr 21, 2009·Veterinary Immunology and Immunopathology·Fumihiko KatakuraTeruyuki Nakanishi
Dec 17, 2008·Developmental and Comparative Immunology·Hideaki TodaTeruyuki Nakanishi
Aug 5, 2008·Veterinary Immunology and Immunopathology·Mohadetheh MoulanaThomas J McConnell
Feb 12, 2008·Developmental and Comparative Immunology·Kyosuke ArakiYuzuru Suzuki
Jul 18, 2006·Developmental and Comparative Immunology·Eva-Stina EdholmMelanie Wilson
Sep 13, 2005·Developmental and Comparative Immunology·Eva BengténMelanie Wilson
Oct 2, 2015·Biology·Teruyuki NakanishiYuta Matsuura
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H ZhouM Wilson
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T B StugeL W Clem
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrey ShirakRamy R Avtalion
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·James L StaffordEva Bengtén
Aug 17, 1999·Cellular Immunology·R J HoganV G Chinchar

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