PMID: 8449248Jan 1, 1993Paper

Cytotoxic activity of Ciona intestinalis (Tunicata) hemocytes: properties of the in vitro reaction against erythrocyte targets

Developmental and Comparative Immunology
N ParrinelloD Parrinello

Abstract

Hemocytes (effectors) of Ciona intestinalis showed a natural cytotoxic capacity (HCA) when assayed in vitro against erythrocytes (targets). Cytotoxic cells lysed, to a variable extent, rabbit (RE), human (A, B, O), guinea pig, and sheep (SE) erythrocytes. Hemocyte cytotoxic activity (HCA) assayed against SE is a calcium-dependent reaction, occurs rapidly (15-30 min), at 25-37 degrees C over a wide range of pH (5.4-8.0). Assays were carried out using: 1) the medium in which hemocytes were maintained, 2) the soluble portion of hemocyte lysates, and 3) debris prepared from hemocyte lysates. Results suggest that HCA is a cell-mediated process that requires effector-target cell contacts. Anti-SE (calcium-dependent) and anti-RE (calcium-independent) agglutinins were also found in the reaction medium, probably released by hemocytes as a consequence of the in vitro experiments. The occurrence of HCA was independent of any allogeneic reaction between mixed hemocytes. Various levels of cytotoxic activity reveal hemocyte specificity.

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Citations

Jan 1, 1995·Developmental and Comparative Immunology·N ParrinelloV Arizza
Jul 1, 1995·Developmental and Comparative Immunology·O KurataY Ikeda
Nov 1, 1995·Developmental and Comparative Immunology·D A Raftos, A Hutchinson
Apr 15, 1994·Annals of the New York Academy of Sciences·C J Bayne, S E Fryer
Jul 11, 2006·Archives of Environmental Contamination and Toxicology·R ThiagarajanH Thilagam
Jan 20, 2011·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Loriano BallarinLucia Manni
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria Rosaria PintoJohn D Lambris
Apr 15, 2005·Molecular Immunology·P EngelmannP Németh

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