Cytotoxic activity of nemorosone in neuroblastoma cells

Journal of Cellular and Molecular Medicine
D Díaz-CarballoH P Reusch

Abstract

Neuroblastoma is the second most common solid tumour during childhood, characterized by rapid disease progression. Most children with metastasized neuroblastoma die despite intensive chemotherapy due to an intrinsic or acquired chemotherapy resistance. Thus, new therapeutic strategies are urgently needed. Here, we demonstrate that the novel compound nemorosone isolated from alcoholic extracts of Clusia rosea resins by reverse phase high pressure liquid chromatography (RP-HPLC) exerts cytotoxic activity in neuroblas-toma cell lines both parental and their clones selected for resistance against adriamycin, cisplatin, etoposide or 5-fluorouracil. Cell cycle studies revealed that nemorosone induces an accumulation in G0/G1- with a reduction in S-phase population combined with a robust up-regulation of p21Cip1. Furthermore, a dose-dependent apoptotic DNA laddering accompanied by an activation of caspase-3 activity was detected. Nemorosone induced a significant dephosphorylation of ERK1/2 in LAN-1 parental cells probably by the inhibition of its upstream kinase MEK1/2. No significant modulation of signal transducers JNK, p38 MAPK and Akt/PKB was detected. The enzymatic activity of immunoprecipitated Akt/PKB was strongly inhibited in ...Continue Reading

References

Jan 1, 1979·International Journal of Radiation Oncology, Biology, Physics·G G Steel, M J Peckham
Sep 14, 1992·Biochimica Et Biophysica Acta·M Schwab
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A Wenzel, M Schwab
Mar 1, 1997·Journal of Pediatric Hematology/oncology·G M BrodeurP S White
Oct 6, 1999·Proceedings of the Society for Experimental Biology and Medicine·K E Boyd, P J Farnham
Jul 13, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M Meyerson
Feb 27, 2001·Japanese Journal of Cancer Research : Gann·T SugimotoS Hattori
Mar 15, 2001·Current Opinion in Cell Biology·D Durocher, S P Jackson
May 31, 2001·Phytochemistry·O Cuesta-RubioJ Cardenas
Jun 12, 2001·Current Opinion in Neurobiology·A Patapoutian, L F Reichardt
Jun 18, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Osmany Cuesta-RubioJorge Cárdenas
Jul 3, 2002·Nature Reviews. Cancer·Igor Vivanco, Charles L Sawyers
Dec 25, 2003·International Journal of Clinical Pharmacology and Therapeutics·D Díaz-CarballoR A Hilger
Mar 17, 2004·Biochemical and Biophysical Research Communications·Fumi Takahashi-YanagaToshiyuki Sasaguri
Apr 28, 2005·Current Topics in Medicinal Chemistry·Eli M WallaceKevin Koch
Jun 17, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shira YaariYoel Kloog
Nov 17, 2005·Oncogene·Jin Q ChengSanto V Nicosia
Jun 26, 2007·Lancet·John M MarisSusan L Cohn
Nov 2, 2007·International Journal of Cancer. Journal International Du Cancer·Jo VandesompeleNadine Van Roy
Nov 3, 2007·Surgical Oncology·Titilope A Ishola, Dai H Chung

❮ Previous
Next ❯

Citations

Jun 14, 2013·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Shailendra Kapoor
Dec 28, 2010·Canadian Journal of Physiology and Pharmacology·Ada PopoloPinto Aldo
Nov 4, 2010·Mitochondrion·Gilberto L Pardo-AndreuCarlos Curti
Aug 12, 2015·Journal of Experimental & Clinical Cancer Research : CR·David Díaz-CarballoDirk Strumberg
Feb 5, 2014·Chemico-biological Interactions·Felippe H Z ReisLuciane C Alberici
Sep 18, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·David Díaz-CarballoDirk Strumberg
Mar 26, 2015·Anais Da Academia Brasileira De Ciências·Maria Carolina AnholetiMaria Auxiliadora C Kaplan
Oct 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tianqiao YongDan Zuo
Mar 31, 2021·Molecular Metabolism·Riya ShresthaFrances L Byrne
Feb 21, 2018·Chemical Reviews·Xing-Wei YangGang Xu
Jul 1, 2015·Organic Letters·Brian A SparlingMatthew D Shair

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence activated cell sorting
FACS
electrophoresis
ELISA
Assay
immunoprecipitation
nuclear magnetic resonance
NMR

Software Mentioned

Millennium

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Clinical Pharmacology and Therapeutics
D Díaz-CarballoH P Reusch
International Journal of Clinical Pharmacology and Therapeutics
D Díaz-CarballoRalf A Hilger
© 2021 Meta ULC. All rights reserved