Cytotoxic activity of sunitinib and everolimus in Caki-1 renal cancer cells is accompanied by modulations in the expression of apoptosis-related microRNA clusters and BCL2 family genes

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Emmanuel I PapadopoulosAndreas Scorilas

Abstract

Sunitinib and everolimus are two of the antineoplastic agents indicated for the management of metastatic renal cancer. Although both of the above compounds were primarily designed as antiangiogenic factors, preclinical studies claim that these drugs can also trigger apoptosis. Herein, we sought to evaluate the cytotoxic activity of sunitinib and everolimus against renal cancer cells Caki-1 and moreover to assess their impact on the expression levels of three BCL2 family members and three apoptosis-related microRNA clusters upon incubation with the drugs or following recovery from treatment. The cytotoxic effect of sunitinib and everolimus on Caki-1 cells' viability was estimated by the MTT assay, while cleaved PARP, assayed via Western Blotting, served as a marker of programmed cell death. As for the expression levels of the BCL2 family members BCL2, BAX and BCL2L12 and those of the mature microRNAs of the miR-183/96/182, miR-143/145, and miR-15a/16 clusters, they were quantified via real-time PCR. Our results showed that both agents induced a time- and dose-dependent decrease in cell viability and promoted cleavage of PARP. In parallel, significant modulations were observed in the expression levels of miR-145, miR-15a, and miR...Continue Reading

References

Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L PegoraroC M Croce
Sep 26, 2001·Experimental Cell Research·C SoldaniA I Scovassi
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Sep 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amelia CimminoCarlo M Croce
Mar 15, 2006·Critical Reviews in Clinical Laboratory Sciences·Hellinida Thomadaki, Andreas Scorilas
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Sep 2, 2008·The Lancet Oncology·Justin A CallD Ross Camidge
Jan 30, 2010·Journal of Cellular Physiology·Subbaya Subramanian, Clifford J Steer
Jul 14, 2010·Biochimica Et Biophysica Acta·Fazli WahidYou Young Kim
Dec 24, 2010·Cell Communication and Signaling : CCS·Ganta Vijay ChaitanyaPhanithi Prakash Babu
Jul 19, 2011·Hematology/oncology Clinics of North America·Lianjie Li, William G Kaelin
Apr 13, 2012·Nucleic Acids Research·Mohit SachdevaYin-Yuan Mo
May 23, 2012·Expert Opinion on Pharmacotherapy·Lori Wood
May 25, 2012·International Journal of Urology : Official Journal of the Japanese Urological Association·Tomoaki FujiokaUNKNOWN Committee for Establishment of the Clinical Practice Guideline for the Management of Renal Cell Carcinoma and the Japanese U
Nov 20, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·B EscudierUNKNOWN ESMO Guidelines Working Group
Nov 10, 2012·International Journal of Biological Sciences·Zofia WotschofskyKlaus Jung

❮ Previous
Next ❯

Citations

May 18, 2016·International Journal of Molecular Sciences·Simona GranataGianluigi Zaza
Oct 12, 2019·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Manal A EmamMarwa Ga Hegazy
Mar 24, 2018·Molecular Medicine Reports·Liyan DuAilin Song
Feb 12, 2017·BMC Complementary and Alternative Medicine·S KhazaeiP Ismail
Jan 14, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·A ToliosT Mohr

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Tao XiangShi-Xuan Wang
© 2021 Meta ULC. All rights reserved