Cytotoxic and antibacterial effect of a red propolis mouthwash, with or without fluoride, on the growth of a cariogenic biofilm

Archives of Oral Biology
Mariana Leonel MartinsAndréa Fonseca-Gonçalves

Abstract

To evaluatein vitro the antibacterial activity, the antibiofilm effect and the cytotoxic potential of mouthwashes containing Brazilian red propolis with or without fluoride. The minimum inhibitory and bactericidal concentrations (MIC and MBC) against S. mutans, S. sanguinis, S. salivarius and L. casei were determined for RPE mouthwashes. A cariogenic biofilm with the aforementioned bacteria was formed over cellulose membrane disks (N = 30, 13 mm), which were submitted for 1 min to the following mouthwashes: plain mouthwash base; 0.05% NaF; 0.8% RPE; 0.8% RPE + 0.05% NaF and 0.12% chlorhexidine (CHX). The bacterial viability and the production of extracellular polysaccharide (EPS) were measured. Cytotoxic potential of the mouthwashes was also evaluated. For bacterial viability and EPS production, Mann-Withney and one-way ANOVA tests were performed followed by Tukey, with results considered significant when p ≤ 0.05. MIC and MBC values of RPE mouthwashes ranged from 7.44 to 29.76 mg/mL and from 7.44 to ≥59.52 mg/mL, respectively, presenting better action against S. salivarius. RPE mouthwashes showed 44% of viable cells after 1 min of contact with fibroblasts. RPE (7.74) had the greatest reduction of viable total microorganisms an...Continue Reading

References

Feb 1, 1985·Toxicology Letters·E Borenfreund, J A Puerner
Aug 27, 2003·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Alberto dos Santos PereiraFrancisco Radler de Aquino Neto
Mar 3, 2005·Journal of Agricultural and Food Chemistry·Adriana FarahPeter R Martin
Jan 19, 2006·Journal of Agricultural and Food Chemistry·Adriana FarahPeter R Martin
Oct 29, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Andreas DaugschYong K Park
Jul 27, 2010·Brazilian Oral Research·Renzo Alberto Ccahuana-Vásquez, Jaime Aparecido Cury
Oct 7, 2010·Journal of Dental Research·N Takahashi, B Nyvad
Aug 8, 2015·BioMed Research International·Jorge Jesús VelozLuis A Salazar
Oct 16, 2015·BMC Complementary and Alternative Medicine·Izabel Cristina Gomes de MendonçaFrancisco Stefânio Barreto
Feb 4, 2016·European Journal of Medicinal Chemistry·Irlan Almeida FreiresPedro Luiz Rosalen
Feb 13, 2016·Archives of Oral Biology·Julia Gabiroboertz CardosoAndréa Gonçalves Antonio
Feb 27, 2016·Journal de mycologie médicale·I A FreiresP L Rosalen
Jul 13, 2016·Journal of the Science of Food and Agriculture·Chuanyi PengXiaochun Wan
Jun 8, 2017·Brazilian Oral Research·Mariana Passos DE LucaPedro Luiz Rosalen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.