PMID: 15348346Sep 7, 2004Paper

Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages - Implication of caspase-3 in the apoptotic pathway

Journal of Materials Science. Materials in Medicine
Isabelle CatelasOlga L Huk

Abstract

The aim of this study was to evaluate the cytotoxic and apoptotic effects of cobalt and chromium ions on macrophages in vitro, and analyze the implication of caspase-3 in the apoptotic pathway. J774 mouse macrophages (5 x 10(5) cells/ml) were exposed for up to 24 h to 0-10 ppm Co2+ and 0-500 ppm Cr3+. The cytotoxic effect of ions was measured by Trypan blue exclusion. DNA analysis on agarose gel was used as a specific test for detection of DNA fragmentation into oligonucleosomes that occurs in apoptotic cells. The proteolytic cleavage of poly(ADP-ribose)polymerase (PARP), closely associated with the induction of apoptosis, was also analyzed along with the appearance of the active fragment of caspase-3, implicated in several apoptosis pathways. Results demonstrated that both Co2+ and Cr3+ ions induce macrophage mortality in a dose-dependent manner. However, Co2+ is more toxic inducing a cell mortality up to 28% with only 10 ppm vs. 37% with 500 ppm of Cr3+. DNA analysis demonstrated that both Co2+ and Cr3+ ions induce DNA fragmentation, between 6-10 ppm Co2+ and 250-500 ppm Cr3+ after 24 h incubation. PARP cleavage and the appearance of caspase-3 active fragment were observed after 6 h with both Co+ and Cr3+ ions, with a stronge...Continue Reading

Citations

Nov 30, 2010·Acta Orthopaedica·Brian M DevittJohn M O'Byrne
Aug 30, 2014·Tissue Engineering. Part a·Maria M AzevedoMolly M Stevens
Jun 16, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephen J BaskeyIsabelle Catelas
Jan 21, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Isabelle CatelasPaul E Beaulé
Oct 8, 2014·Journal of Orthopaedic Surgery and Research·Arihiko KanajiKotaro Sena
Aug 26, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zeina SalloumIsabelle Catelas
Jun 22, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Emmanuel GibonStuart B Goodman
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