Cytotoxic and biomechanical effects of clinical dosing schemes of paclitaxel on neurons and cancer cells.

Cancer Chemotherapy and Pharmacology
Chou-Ching K LinMing-Shaung Ju

Abstract

Chemotherapy-induced peripheral neuropathy often results in a reduction in drug dose. However, the serum level of anticancer drugs varies with time after intravenous infusion, and this factor has seldom been considered in previous in vitro studies. The goals of this study were to build an automatic dosage control system and to evaluate the influence of drug infusion rate on the cells. Neurons and melanoma cells were used as the samples. The 3-h (average and peak concentration: 0.024 and 0.287 μM) and 24-h infusion (average and peak concentration: 0.020 and 0.042 μM) schemes were investigated. For evaluations, cell indentation tests by an atomic force microscope, serial immunofluorescent images, and cell viability analysis was performed. For the neurons, Young's modulus first increased and then remained unchanged in the 3-h scheme, but was stationary throughout the observation period in the 24-h scheme. For the cancer cells, Young's modulus increased in both infusion schemes, and the increase was larger in the 3-h scheme. Morphologically, axons swelled and shortened, and the number of their branches decreased in the 3-h scheme. In contrast, there was only slowed growth of axons without obvious morphological changes in the 24-h s...Continue Reading

References

Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
Jan 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·L GianniM J Egorin
Dec 1, 1994·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·E A EisenhauerN Colombo
Apr 16, 1998·Environmental Health Perspectives·G J HarryH A Tilson
Aug 9, 2006·Nature Clinical Practice. Oncology·Richard Simon, Larry Norton
Dec 3, 2009·Journal of the National Comprehensive Cancer Network : JNCCN·Michael D StubblefieldJamie H Von Roenn
Nov 7, 2009·Brazilian Oral Research·Ricardo Carneiro BorraPriscila Maria Andrade
Aug 31, 2010·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·Chunming LiMartin D Fox
Jun 1, 1986·IEEE Transactions on Pattern Analysis and Machine Intelligence·J Canny
Sep 13, 2011·Critical Reviews in Oncology/hematology·Andreas A ArgyriouGuido Cavaletti
Feb 18, 2014·Biochimica Et Biophysica Acta·Yu M EfremovK V Shaitan
Apr 29, 2015·Journal of Biomechanics·Cheng-Tao ChangMing-Shaung Ju
Aug 19, 2015·Frontiers in Cellular Neuroscience·Kate M O'NeillBonnie L Firestein
Sep 1, 2015·Journal of Biomechanics·Brian G Bober, Sameer B Shah
Nov 26, 2015·Scientific Reports·Kwang-Min KimG Tayhas R Palmore
Nov 30, 2016·Experimental Neurology·Erica L Gornstein, Thomas L Schwarz

❮ Previous
Next ❯

Citations

Nov 26, 2020·International Journal of Molecular Sciences·Andrzej KubiakMałgorzata Lekka
May 1, 2021·Cells·Gaël RunelIngrid Masse

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.