Cytotoxic and DNA-damaging effects of diterpenoid quinones from the roots of Salvia officinalis L. on colonic and hepatic human cells cultured in vitro

Basic & Clinical Pharmacology & Toxicology
Darina SlamenováLadislava Wsólová

Abstract

Three diterpenoid quinones (royleanone- SAR 3, horminone- SAR 26, and acetyl horminone- SAR 43) isolated from the roots of Salvia officinalis L. were tested for their cytotoxic and DNA-damaging activity in human colon carcinoma cells Caco-2 and human hepatoma cells HepG2 cultured in vitro. Cytotoxicity was measured by the trypan blue exclusion technique and induction of apoptosis was evaluated by flow immunofluorocytometry after 30-300 min. exposure of HepG2 and Caco-2 cells to diterpenoid quinones and following 24 hr post-incubation in the culture medium. Induction of DNA breaks was measured after 60 min. exposure of cells to different concentrations of the compounds studied by the alkaline elution of DNA and by the Comet assay. Though all the quinones tested decreased the viability of the cells studied proportionally to the concentration and to the time of treatment (cytotoxicity= 30-60%), the increased level of apoptotic nuclei comparable to the level of apoptotic nuclei induced by a topoisomerase I inhibitor was proved only in HepG2 cells treated with 1x10(-4) mol/l SAR 26 or SAR 43. Either no or marginal increase of the level of apoptotic nuclei was observed in SAR 3-treated HepG2 cells and in SAR 3-, SAR 26- or SAR 43-tre...Continue Reading

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Citations

Jul 15, 2011·Acta Crystallographica. Section E, Structure Reports Online·Hoong-Kun FunChatchanok Karalai
Nov 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Siu-Lung Chan, John H K Yeung
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Jul 15, 2021·Natural Products and Bioprospecting·Patrick O SakyiSamuel K Kwofie
Feb 26, 2015·Journal of Natural Products·Onat Kadioglu, Thomas Efferth

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