Cytotoxic and genotoxic characterization of aluminum and silicon oxide nanoparticles in macrophages

Dental Materials : Official Publication of the Academy of Dental Materials
Masanori Hashimoto, Satoshi Imazato

Abstract

Although aluminum oxide and silicon oxide nanoparticles are currently available as dental materials, there is a lack of basic information concerning their biocompatibility. This study evaluates the biological responses of cultured macrophages (RAW264) to aluminum oxide (Al2O3NPs) and silicon oxide nanoparticles (SiO2NPs) by analyzing cytotoxicity and genotoxicity. The nanoparticles are amorphous and spherical, with diameters of 13 nm for the Al2O3NPs and 12 nm for the SiO2NPs. The cultured RAW264 are exposed to the nanoparticles (NPs) and examined for cytotoxicity using the WST-8 cell viability and Hoechst/PI apoptosis assay, for genotoxicity by micronucleus analysis, for changes in nuclear shape (deformed nuclei) and for comet assay using confocal microscopy, and micromorphological analysis is done using scanning and transmission electron microscopes. Nuclei and DNA damage because of exposure to both types of NPs is observed by inmunostaining genotoxicity testing. The cytotoxicity and genotoxicity are well correlated in this study. Numerous NPs are observed as large aggregates in vesicles, but less or nonexistent NP internalization is seen in the nucleus or cytoplasm. These morphological results suggest that a primary cause of...Continue Reading

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Citations

Mar 11, 2016·Journal of Hazardous Materials·Sung Hee Joo, Dongye Zhao
Mar 25, 2017·Journal of Applied Toxicology : JAT·Qinli ZhangQiao Niu
Aug 29, 2018·Nanomedicine·Liangjiao ChenLongquan Shao
May 5, 2018·International Journal of Toxicology·Giray Buğra Akbaba, Hasan Türkez
Jun 3, 2017·Archives of Toxicology·Sivakumar MurugadossPeter H Hoet
Aug 6, 2020·Toxics·Jorge Humberto Limón-PachecoMarisol Orozco-Ibarra
Aug 11, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Niyou WangA Senthil Kumar
Dec 29, 2020·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Natalia Almeida BastosGrace Mendonca De Souza
Nov 1, 2020·Journal of Materials Science. Materials in Medicine·Jessica Zablocki da LuzFrancisco Filipak Neto
Aug 25, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Marcel F KunrathMaria M Campos
Sep 19, 2021·Biological Trace Element Research·Sung-Hyeuk ParkJong-Choon Kim

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