Cytotoxic and genotoxic effects of cleistanthin B in normal and tumour cells

Mutagenesis
C PrabhakaranG Shanmugam

Abstract

Cleistanthin B, one of the toxic constituents of Cleistanthus collinus, was found to be cytotoxic to normal and tumour cells. In comparison with normal cells, tumour cells were sensitive to lower doses of toxin. The 50% growth inhibition (GI50) values for normal cell lines were from 2 x 10(-5) to 4.7 x 10(-4) M and for tumour cells the values ranged from 1.6 x 10(-6) to 4 x 10(-5) M. Short exposure (30 min) of Chinese hamster ovary (CHO) cells to cleistanthin B at 1-6 micrograms/ml resulted in extensive chromatid and isochromatid breaks and gaps. However there was no significant increase in cell death and DNA strand breaks in cells treated under the above conditions. Cleistanthin B induced micronucleus formation in cultured lymphocytes in a dose-dependent manner. CHO cells treated with high doses of cleistanthin B showed a decrease in cell viability and a concomitant increase in DNA strand-breaks. The cell death appears to be due to apoptosis since nucleosome-like ladders were observed in the treated cells when the DNA was electrophorized in agarose gels.

Citations

Jan 29, 2000·Mutation Research·C P PradheepkumarG Shanmugam
Jul 13, 2012·Journal of Emergencies, Trauma, and Shock·Anugrah Chrispal
Oct 16, 2014·Journal of Basic and Clinical Pharmacy·R P PriyadharsiniR Raveendran
Jul 23, 2003·Journal of Toxicology. Clinical Toxicology·Shashiraj EswarappaMabel Vasnaik
Aug 10, 2011·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Kavitha Priya KettimuthuAnup Ramachandran
Jun 18, 2014·Journal of Natural Products·Yulin RenA Douglas Kinghorn
Jul 22, 2014·Journal of Forensic Sciences·Siddhartha DasRavichandran Muthusamy
Oct 25, 2016·Journal of Traditional and Complementary Medicine·Vipul R ThummarRamasamy Raveendran
Feb 6, 2019·Natural Product Research·Shi-Rui FanXiao-Jiang Hao

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