Cytotoxic and genotoxic evaluation of different synthetic amorphous silica nanomaterials in the V79 cell line

Toxicology and Industrial Health
Y GuichardC Darne

Abstract

The nature of occupational risks and hazards in industries that produce or use synthetic amorphous silica (SAS) nanoparticles is still under discussion. Manufactured SAS occur in amorphous form and can be divided into two main types according to the production process, namely, pyrogenic silica (powder) and precipitated silica (powder, gel or colloid). The physical and chemical properties of SAS may vary in terms of particle size, surface area, agglomeration state or purity, and differences in their toxicity potential might therefore be expected. The aim of this study was to compare the cytotoxicity and genotoxicity of representative manufactured SAS samples in Chinese hamster lung fibroblasts (V79 cells). Five samples from industrial SAS producers were evaluated, that is, two pyrogenic SAS powders (with primary particle sizes of 20 nm and 25/70 nm), one precipitated SAS powder (20 nm) and two precipitated SAS colloids (15 and 40/80 nm). V79 cell cultures were treated with different concentrations of SAS pre-dispersed in bovine serum albumin -water medium. Pyr (pyrogenic) 20, Pre (precipitated) 20 and Col (colloid) 15 significantly decreased the cell viability after 24 h of exposure, whilst Pyr 25/70 and Col 40/80 had negligible...Continue Reading

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Citations

Nov 28, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Luisana Di CristoEnrico Bergamaschi
Mar 7, 2019·Nature Nanotechnology·Martin MiernickiAntonia Praetorius
Jul 1, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Ziyuan LiZhongjun Du
Sep 30, 2017·Nanotoxicology·Carole SeidelYves Guichard
Jun 3, 2017·Archives of Toxicology·Sivakumar MurugadossPeter H Hoet
Jan 17, 2018·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS)Claude Lambré
Dec 10, 2020·Journal of Hazardous Materials·Caixia GuoYanbo Li
Nov 28, 2020·International Journal of Nanomedicine·Xuemeng DongZhiwei Sun

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Methods Mentioned

BETA
transmission electronic microscopy
dynamic light scattering
atomic emission spectrometry
FCS
Assay

Software Mentioned

Statgraphics Centurion

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