Cytotoxic cells induced during lymphocytic choriomeningitis virus infection of mice: natural killer cell activity in cultured spleen leukocytes concomitant with T-cell-dependent immune interferon production.

Infection and Immunity
R M Welsh, W F Doe

Abstract

The characteristics and specificities of spleen and peritoneal cytotoxic cells generated during lymphocytic choriomeningitis virus (LCMV) infection of C3H/St mice were examined. Activated natural killer (NK) cell activity was identified in fresh leukocyte populations from the 2nd to 8th days postinfection, whereas virus-specific cytotoxic T-cell activity was detected from the 6th to 14th days. When leukocytes were cultured overnight at 37 degrees C before assay, T-cell activity was still observed, but nonspecific activated NK cell-like cytotoxicity was only detected on the 6th and to a lesser degree the 8th day postinfection. Overnight culture of leukocytes taken earlier in the infection eliminated their NK cell activity. Similar activities were seen with spleen cell, plastic-adherent peritoneal cell, and nonadherent peritoneal cell populations. The virus-specific cytotoxicity observed with adherent peritoneal cells was due to contamination with cytotoxic T cells, as shown by H-2-restricted cytotoxicity and sensitivity to anti-theta antibody and complement. The nonspecific cultured day 6 effector cell from either the spleen or peritoneum displayed killing specificities and other physical properties identical to those of activat...Continue Reading

References

Aug 1, 1978·The Journal of Experimental Medicine·W F Doe, P M Henson
Sep 15, 1977·International Journal of Cancer. Journal International Du Cancer·M E NunnH T Holden
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E S VitettaF Jacob
Aug 15, 1975·International Journal of Cancer. Journal International Du Cancer·R B HerbermanD H Lavrin
Jun 1, 1973·The Australian Journal of Experimental Biology and Medical Science·R V Blanden, C A Mims
May 15, 1980·International Journal of Cancer. Journal International Du Cancer·R W Kiessling, R M Welsh

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Citations

Jan 1, 1982·Medical Microbiology and Immunology·C A Biron, R M Welsh
Aug 1, 1987·Scandinavian Journal of Immunology·S J WoodsC J Pfau
Jan 15, 1981·International Journal of Cancer. Journal International Du Cancer·L A Kunkel, R M Welsh

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