Cytotoxic Constituents from the Sclerotia of Poria cocos against Human Lung Adenocarcinoma Cells by Inducing Mitochondrial Apoptosis

Cells
Seulah LeeKi Hyun Kim

Abstract

Previous studies have revealed the antitumor potential of Poria cocos Wolf against a broad spectrum of cancers. However, the biological activity of P. cocos against lung cancer, which is known as the leading cause of cancer mortality worldwide, and its underlying chemical and molecular basis, remain to be investigated. We aimed to evaluate the in vitro cytotoxicity of P. cocos toward human lung adenocarcinoma cells with different p53 statuses, to identify the bioactive constituents of P. cocos, and explicate the molecular mechanisms underlying the cytotoxicity of these constituents in human lung adenocarcinoma cells. An EtOH extract of the sclerotia of P. cocos exhibited cytotoxicity toward four human lung cancer cell lines: A549, H1264, H1299, and Calu-6, regardless of their p53 status. Chemical investigation of the extract resulted in the isolation of two triterpenoids, dehydroeburicoic acid monoacetate (1) and acetyl eburicoic acid (4); a sterol, 9,11-dehydroergosterol peroxide (2); and a diterpenoid, dehydroabietic acid (3). All of the isolated compounds were cytotoxic to the lung adenocarcinoma cell lines, exhibiting IC50 values ranging from 63.6 μM to 171.0 μM at 48 h of treatment. The cytotoxicity of the extract and the ...Continue Reading

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Citations

Apr 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qin-Qin WangYuan-Zhong Wang
May 31, 2020·The Journal of Antibiotics·Seoung Rak LeeKi Hyun Kim
Apr 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dahae LeeKi Hyun Kim
Oct 20, 2020·Frontiers in Pharmacology·Anzheng NieChunsheng Zhu
Aug 19, 2020·Journal of Microbiology and Biotechnology·Seoung Rak LeeKi Hyun Kim
Dec 31, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Anith M M SairiSumaiyah Abdullah

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Methods Mentioned

BETA
Assay
column chromatography

Software Mentioned

GraphPad
GraphPad Prism

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