PMID: 9639560Jun 26, 1998Paper

Cytotoxic effect of HIV-1 coat glycoprotein gp120 on human neuroblastoma CHP100 cells involves activation of the arachidonate cascade

The Biochemical Journal
M MaccarroneA Finazzi Agrò

Abstract

HIV type-1 (HIV-1) coat glycoprotein gp120 causes necrotic death in human neuroblastoma CHP100 cells. Here, we investigated the possible role of the arachidonate cascade and membrane peroxidation in gp120-induced cell necrosis. It is shown that gp120 increases the intracellular concentrations of prostaglandin E2 and leukotriene B4 by up-regulating the activity and expression of the arachidonate-metabolizing enzymes prostaglandin H synthase and 5-lipoxygenase respectively. Consistent with this observation, selective inhibitors of prostaglandin H synthase (i.e. indomethacin) and 5-lipoxygenase (i.e. MK886 and caffeic acid) protected CHP100 cells against gp120-induced necrosis. Treatment with gp120 also enhanced membrane lipid peroxidation and this may be implicated in the execution of cell damage. Interestingly, incubation with exogenous nitric oxide (NO) mimicked the effects of gp120 on necrotic death of CHP100 cells and activation of prostaglandin H synthase and 5-lipoxygenase. This suggests that NO might participate in the mechanism by which gp120 activates the arachidonate cascade.

Citations

Oct 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M J CoffeyM Peters-Golden
Oct 2, 2002·Journal of Neurochemistry·Mauro MaccarroneAlessandro Finazzi-Agrò
Jun 14, 2017·Mediators of Inflammation·Paolo GelosaLaura Castiglioni
Sep 22, 1999·European Journal of Biochemistry·M MacCarroneA F Agrò
Dec 12, 2001·Clinical and Experimental Immunology·J GosselinP Borgeat
Aug 9, 2001·Journal of Neuroscience Research·M NavarraG Di Renzo
Jun 2, 2000·Biochemical and Biophysical Research Communications·M MaccarroneA Finazzi-Agrò
Sep 7, 1999·Journal of the American Society of Nephrology : JASN·M MaccarroneA Finazzi-Agrò
Jul 6, 2000·Japanese Journal of Pharmacology·K SugayaH Manev
Aug 30, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Mauro MaccarroneMassimo Taccone-Gallucci
Mar 24, 2004·Free Radical Biology & Medicine·Letizia PalombaOrazio Cantoni
Apr 28, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·M MaccarroneM Taccone-Gallucci

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