Cytotoxic effect of Montivipera bornmuelleri's venom on cancer cell lines: in vitro and in vivo studies

PeerJ
Carol HaddoubMarc C Karam

Abstract

Montivipera bornmuelleri's venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCA-induced murine fibrosarcoma cell lines to M. bornmuelleri's venom and its effect on tumor growth in vivo. The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 µg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri's venom in vitro which, however, does not translate to an anticancer action in vivo.

References

Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Oct 1, 1989·Annals of Internal Medicine·R O Dillman
Oct 4, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Benjamin D EhstMarc K Jenkins
Jan 7, 2004·Nature Reviews. Cancer·Glenn Dranoff
Jul 16, 2005·Biomaterials·Maud B Gorbet, Michael V Sefton
Jul 20, 2005·Clinical and Experimental Pharmacology & Physiology·Sheng-Huei YangShinne-Ren Lin
Jan 30, 2007·Journal of Ethnopharmacology·Anindita DebnathAparna Gomes
Apr 12, 2007·International Anesthesiology Clinics·Jun-Ming Zhang, Jianxiong An
Sep 14, 2011·The Journal of Clinical Investigation·Tangying LuDmitry Gabrilovich
Jan 1, 2011·Cancers·Sylvia Lee, Kim Margolin
Apr 1, 2014·BioMed Research International·Leonardo A CalderonAndreimar M Soares
Nov 6, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Cássio Prinholato da SilvaSuely V Sampaio
Aug 12, 2016·Clinical Pharmacology and Therapeutics·J S McCune
Oct 30, 2016·Anticancer Research·Ammar SukariLawrence G Lum
Jan 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Emanuelly Bernardes-OliveiraJanaina Cristiana de Oliveira Crispim
Jan 13, 2017·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Saly SawanMarc Karam
Dec 17, 2017·Oncotarget·Rui MaHang Fai Kwok
May 16, 2018·Toxicon : Official Journal of the International Society on Toxinology·Harald KerkkampDaniëlle Vlecken
Oct 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamad RimaZiad Fajloun
Nov 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Steve SwensonFrancis S Markland
Jan 27, 2019·Investigational New Drugs·Narine GhazaryanElsa Logarinho
May 28, 2019·International Journal of Biological Macromolecules·Fernanda Van Petten de Vasconcelos AzevedoVeridiana de Melo Rodrigues

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Methods Mentioned

BETA
Assay
flow cytometry

Software Mentioned

GraphPad Prism
GraphPad

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