PMID: 20650086Jun 1, 1995Paper

Cytotoxic effect of prolamin-derived peptides on in vitro cultures of cell line Caco-2: Implications for coeliac disease

Toxicology in Vitro : an International Journal Published in Association with BIBRA
C GiovanniniM De Vincenzi

Abstract

The cytotoxic effects of various prolamin-derived peptides on Caco-2 cells were investigated by measuring the alterations of several parameters at different stages of cell differentiation. The PT digest of bread wheat was active in inhibiting cell proliferation (by about 50%), whereas the other digests from durum wheat, maize and bovine serum albumin (BSA) did not affect the proliferating activity of cells. Compared with the control, colony-forming ability was inhibited by 20% by treatment with cereals that are toxic in coeliac disease (bread wheat, rye, oats and barley). BSA and maize peptides are devoid of this in vitro effect. However, the decrease in alkaline phosphatase activity during Caco-2 cell differentiation was observed in the presence of bread wheat. This could be due to slowing down of the enterocytic differentiation of cells that are susceptible to interaction with toxic peptides. Therefore, long-term cultures of Caco-2 cells constitute a useful in vitro model to assess the ability of cereal proteins to damage the coeliac small intestine.

References

Dec 1, 1985·Journal of Pediatric Gastroenterology and Nutrition·S AuricchioV Silano
Sep 1, 1984·The American Journal of Physiology·E GrassetJ F Desjeux
Jun 15, 1984·Biochemical and Biophysical Research Communications·S AuricchioV Silano

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Citations

Nov 15, 2003·Toxicology Letters·Luca ElliMaria Teresa Bardella
Apr 20, 2000·Toxicology·C GiovanniniM De Vincenzi
Jul 12, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E DolfiniM T Bardella
Oct 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C GiovanniniM De Vincenzi
Aug 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A NaraiM Shimizu
Nov 15, 2006·Clinical and Experimental Immunology·W SaklyS El Alaoui
Oct 5, 2011·Molecular Nutrition & Food Research·Paolo BergamoMauro Rossi
May 19, 2012·Nutrients·Gianna FerrettiLetizia Saturni
Apr 18, 2019·International Journal of Molecular Sciences·Antonella OrlandoFrancesco Russo
Sep 24, 2020·Plant Foods for Human Nutrition·Sara Avilés-GaxiolaJ Basilio Heredia

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