Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells.

Journal of Toxicology
Guey-Horng WangChia-Hua Liang

Abstract

Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G(0)/G(1) and G(2)/M phases with a concomitant significantly increased sub-G(1) fraction, suggesting cell death by apoptosis. Extracts of the genus Sinularia thus apparently cause apoptosis of SCC25 and HaCaT cells, and warrant further research investigating the possible antioral cancer compounds in these soft corals.

References

Oct 29, 1976·Science·G D Ruggieri
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R N FabricantG J Todaro
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S MiyauchiK Takada
Aug 29, 1998·Journal of Natural Products·C Y DuhJ H Sheu
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·T L AceretP W Sammarco
Nov 23, 2000·Journal of Natural Products·J SuL Zeng
Mar 14, 2001·Natural Product Reports·D J Faulkner
Mar 21, 2002·Natural Product Reports·D John Faulkner
May 15, 2002·Journal of Ethnopharmacology·L LopézV Maldonado
Oct 25, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T García-GascaL A Salazar-Olivo
Oct 26, 2002·Journal of Natural Products·Guey-Horng WangMichael Y Chiang
Apr 24, 2004·Journal of Natural Products·Atallah F AhmedJyh-Horng Sheu
Sep 23, 2006·Journal of Natural Products·Atallah F AhmedJyh-Horng Sheu

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Methods Mentioned

BETA
Flow Cytometry
Assay

Software Mentioned

WinMDI

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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