Abstract
Human peripheral blood monocytes from normal donors were isolated by differential centrifugation and cultured in vitro in hydrophobic Teflon-coated tissue culture bags. Cells were harvested between 0 and 10 days and tested for their ability to kill schistosomula of Schistosoma mansoni in an in-vitro cytotoxicity assay. Freshly isolated, unstimulated monocytes demonstrated minimal cytotoxic capability. However, this was increased if the cells were pretreated with human recombinant gamma interferon (IFN-gamma), or with specific anti-S. mansoni antiserum. As the monocytes matured in vitro there were marked increases in the levels of antibody-independent killing of schistosomula. Monocytes grown in vitro with IFN-gamma (10(4) u/ml) took 2-3 days to develop almost maximal cytotoxicity (mean 94% kill of schistosomula). In contrast, unstimulated monocytes (no IFN-gamma) took between 5 and 7 days to achieve comparable cytotoxicity (mean 99% kill). Killing of the schistosomula was dependent upon a high effector to target ratio, and was a relatively slow phenomenon in vitro, parasite attrition occurring between 17 and 36 h. Supernatants from cytotoxic macrophages were ineffective in mediating cytotoxicity of the parasite.
References
May 1, 1977·European Journal of Immunology·A CapronH Bazin
Feb 6, 1975·Nature·A CapronH Bazin
May 1, 1986·Parasite Immunology·J E Crabtree, R A Wilson
Sep 4, 1986·Nature·R Philip, L B Epstein
Jan 1, 1986·Immunologic Research·S L James
Jan 1, 1986·Transactions of the Royal Society of Tropical Medicine and Hygiene·D G ColleyM A el Alamy
Jun 1, 1987·Acta Tropica. Supplementum·A E Butterworth
Mar 24, 1988·The New England Journal of Medicine·R B Johnston
May 1, 1985·Parasite Immunology·D J McLaren, S L James
Mar 1, 1972·International Journal for Parasitology·J A Clegg, S R Smithers
May 1, 1969·The Journal of Experimental Medicine·G B Mackaness
Mar 1, 1982·Cellular Immunology·S L JamesA Sher
Jan 1, 1983·Transactions of the Royal Society of Tropical Medicine and Hygiene·A CapronM Joseph
May 1, 1983·Scandinavian Journal of Immunology·A Bøyum
Sep 1, 1983·The Journal of Experimental Medicine·C F NathanB Y Rubin
Jun 1, 1984·Infection and Immunity·S L JamesE J Leonard
Jun 1, 1983·The Journal of Experimental Medicine·M C Veith, A E Butterworth
Nov 15, 1982·Cellular Immunology·S L JamesM S Meltzer
Jan 1, 1987·Parasitology Today·A E Butterworth, P Hagan
Oct 1, 1987·Parasitology Today·I A Clark
Jun 1, 1983·Immunology Today·D O Adams, C F Nathan
Citations
Aug 1, 1990·Atherosclerosis·K L CarpenterM J Mitchinson
Jan 1, 1994·Mediators of Inflammation·D GoldY Keisari
Dec 14, 2011·International Journal for Parasitology·Gabriel RinaldiPaul J Brindley
Nov 1, 1989·Parasite Immunology·K R JonesJ H Playfair
Jan 15, 2014·PLoS Neglected Tropical Diseases·Evaristus Chibunna MbanefoKenji Hirayama
May 13, 1999·Parasite Immunology·F J Van MilligenB A Bokhout
Sep 28, 1999·Tropical Medicine & International Health : TM & IH·M MargueriteA Capron
Aug 1, 1995·European Journal of Immunology·P Couissinier-Paris, A J Dessein