PMID: 2494635Jan 1, 1989Paper

Cytotoxic effects in vitro of human monocytes and macrophages on schistosomula of Schistosoma mansoni

Parasite Immunology
B CottrellA Butterworth

Abstract

Human peripheral blood monocytes from normal donors were isolated by differential centrifugation and cultured in vitro in hydrophobic Teflon-coated tissue culture bags. Cells were harvested between 0 and 10 days and tested for their ability to kill schistosomula of Schistosoma mansoni in an in-vitro cytotoxicity assay. Freshly isolated, unstimulated monocytes demonstrated minimal cytotoxic capability. However, this was increased if the cells were pretreated with human recombinant gamma interferon (IFN-gamma), or with specific anti-S. mansoni antiserum. As the monocytes matured in vitro there were marked increases in the levels of antibody-independent killing of schistosomula. Monocytes grown in vitro with IFN-gamma (10(4) u/ml) took 2-3 days to develop almost maximal cytotoxicity (mean 94% kill of schistosomula). In contrast, unstimulated monocytes (no IFN-gamma) took between 5 and 7 days to achieve comparable cytotoxicity (mean 99% kill). Killing of the schistosomula was dependent upon a high effector to target ratio, and was a relatively slow phenomenon in vitro, parasite attrition occurring between 17 and 36 h. Supernatants from cytotoxic macrophages were ineffective in mediating cytotoxicity of the parasite.

References

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Citations

Aug 1, 1990·Atherosclerosis·K L CarpenterM J Mitchinson
Dec 14, 2011·International Journal for Parasitology·Gabriel RinaldiPaul J Brindley
Nov 1, 1989·Parasite Immunology·K R JonesJ H Playfair
Aug 1, 1995·European Journal of Immunology·P Couissinier-Paris, A J Dessein

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