Cytotoxic effects induced by patulin, sterigmatocystin and beauvericin on CHO-K1 cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Nidhal ZouaouiMaría-José Ruiz

Abstract

Mycotoxins are produced by different genera of fungi; mainly Aspergillus, Penicillium and Fusarium. The natural co-occurrence of beauvericin (BEA), patulin (PAT) and sterigmatocystin (STE) has been proved in feed and food commodities. This study investigates the cytotoxicity of individual and combined mycotoxins BEA, PAT and STE. The cytotoxicity on immortalized ovarian cells (CHO-K1) was evaluated using the MTT assay. After 24, 48 and 72 h, the IC50 values were 2.9 μM for PAT and ranged from 10.7 to 2.2 μM and from 25.0 to 12.5 μM for BEA and STE, respectively. Cytotoxic interactions were assayed by the isobologram method, which provides a combination index (CI) value as a quantitative measure of the three mycotoxin interaction's degree. Binary and tertiary combinations showed a dose dependent effect. At low fraction affected, mycotoxin combinations were synergetic; whereas, at higher fraction affected, the combinations showed additive effect. Our results indicate that the co-occurrence of low concentrations of mycotoxin in food may increase their toxic effects.

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Citations

Sep 8, 2016·Journal of Agricultural and Food Chemistry·Christiane Gruber-DorningerFranz Berthiller
Mar 9, 2018·Molecular Plant Pathology·Christelle El Hajj AssafOlivier Puel
Sep 12, 2018·Drug and Chemical Toxicology·Sevcan MamurDeniz Yüzbaşıoğlu
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Jul 8, 2019·World Journal of Microbiology & Biotechnology·Taoling MinHaifeng Hu
Apr 21, 2020·Toxicology Mechanisms and Methods·Mercedes TaroncherMaria-Jose Ruiz
Nov 13, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Veronica ZingalesMaria-José Ruiz
Nov 4, 2020·Toxins·Alessia BerteroFrancesca Caloni
Oct 10, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Veronica ZingalesMaria-José Ruiz
Sep 6, 2020·Ecotoxicology and Environmental Safety·Bhuvaneshwari ManivannanMikhail Borisover
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sudharsan SadhasivamEdward Sionov
Jun 28, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Veronica ZingalesMaria-José Ruiz
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Oct 12, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Veronica ZingalesElisa Cimetta

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