Cytotoxic effects of diazenes on tumor cells in vitro

Chemotherapy
Dubravka MoskateloM Osmak

Abstract

We have shown previously that diazenecarboxamides (diazenes) were cytotoxic for several tumor cell lines. Their target seems to be the intracellular glutathione (GSH). To improve the solubility and biological activity of these drugs, new compounds have been synthesized. In the present study we examined the cytotoxic effect of six new diazenes: BR-25, UP-39, UP-11, JK-1024, RL-514 and RL-625. The cytotoxicity of diazenes was tested on nine human tumor cell lines: laryngeal carcinoma HEp2 cells, cervical carcinoma HeLa cells, mammary carcinoma MCF-7 cells, breast adenocarcinoma SK-BR-3 cells, and glioblastoma A1235 cells and their four drug-resistant cell lines. Cytotoxicity was determined using the colorimetric MTT assay. The intracellular GSH content (following the treatment with diazenes) was examined spectrophotometrically in human cervical carcinoma cells by the procedure developed by Tietze. Results show that diazene UP-39 was mostly efficient, significantly reducing the cell survival of all nine cell lines examined, including four drug-resistant cell lines. Diazenes UP-11, RL-514 and BR-25 given in the highest concentrations decreased the survival of some examined cell lines, but to less than 50%. Diazenes RL-625 and JK-10...Continue Reading

Citations

Feb 8, 2006·Cell Biology and Toxicology·S JakopecM Osmak
Mar 26, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T CimboraM Osmak
Apr 14, 2006·Chemotherapy·L O'Driscoll, M Clynes
Aug 10, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I Martin-KleinerJ Gabrilovac
Jan 23, 2014·European Journal of Medicinal Chemistry·Davie CappoenJanez Košmrlj
Dec 12, 2003·World Journal of Gastroenterology : WJG·Jian ZhugeYing-Nian Yu
Oct 17, 2008·Journal of Combinatorial Chemistry·Damijana Urankar, Janez Kosmrlj

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