Cytotoxic effects of mono- and di-rhamnolipids from Pseudomonas aeruginosa MR01 on MCF-7 human breast cancer cells

Colloids and Surfaces. B, Biointerfaces
Kobra RahimiShahla Mohammad Ganji

Abstract

Rhamnolipids produced by P. aeruginosa MR01 were fractionated into mono- and di-rhamnolipids, and their dominant congeners, Rha-C10-C10 and Rha-Rha-C10-C10, were shown by mass spectrometry. Minimum surface tensions and critical micelle concentrations (CMC) were determined as "≃34 mN/m; ≃26.17 mg/l;" and "≃29 mN/m; ≃29.63 mg/l" for mono- and di-rhamnolipids, respectively. Spectrophotometry measurements provided a close approximation of CMC. Contact angle and diameter of wet area were determined for rhamnolipid-containing drops on hydrophobic paper to display their capability for alteration of surface wettability. Wet area measurement is a simple, reliable method not requiring a Drop Shape Analyzer. Cell viabilities determined by MTT assay showed a decline in a dose-dependent manner and estimated IC50 values were 25.87 μg/ml and 31.00 μg/ml for mono- and di-rhamnolipids treating MCF-7 cells for 48 h. Morphological observations using the inverted phase-contrast microscopy and fluorescence microscopy via Hoechst staining revealed the apoptotic characteristics in treated MCF-7 cells. The semi-quantitative RT-PCR method demonstrated that expression of the p53 gene in mRNA levels significantly (P < 0.05) increased when treated with 30...Continue Reading

Citations

Jul 28, 2020·Pharmaceutics·Ioannis AnestopoulosMihalis I Panayiotidis
Mar 7, 2021·International Journal of Molecular Sciences·Georgia-Persephoni VoulgaridouAglaia Pappa
Jun 14, 2021·Seminars in Cancer Biology·Muhammad Jameel Mughal, Hang Fai Kwok
Oct 10, 2020·Current Drug Metabolism·Shubham ThakurSubheet Kumar Jain
Dec 1, 2020·Organic Letters·Lei Wang, Todd L Lowary

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