PMID: 20650151Oct 1, 1995Paper

Cytotoxic effects of sublethal concentrations of malachite green in isolated hepatocytes from rainbow trout (Oncorhynchus mykiss)

Toxicology in Vitro : an International Journal Published in Association with BIBRA
T Zahn, T Braunbeck

Abstract

The fish therapeutic dye malachite green (N-[4-[bis]4-(dimethylamino)phenyl[phenyl]-methylene]) was tested for sublethal cytotoxic effects at concentrations of 0, 0.01, 0.1 and 1 mg/litre. Isolated hepatocytes of rainbow trout (Oncorhynchus mykiss) were used to evaluate its cytotoxicity in a subcellular system and compare in vitro effects with those of in vivo experiments. Whereas conventional viability tests [trypan blue exclusion, lactate dehydrogenase (LDH) release]failed to detect any acute toxic effect by malachite green exposure, electron microscopy revealed time- and dose-dependent responses of isolated hepatocytes with first reactions after 1-day exposure to 0.01 mg/litre. Whereas hepatocellular nuclei took a more irregular shape, cytoplasmic changes comprised an increase in heterogeneity of mitochondrial shape, closer association of mitochondria with cisternae of the rough endoplasmic reticulum (RER), fractionation, dilation and vesiculation of RER, formation of cytoplasmic membrane whorls, glycogen bodies and induction of myelinated bodies and cytoplasmic vacuoles. In agreement with conclusions drawn from in vivo experiments, in vitro data provided evidence for nuclei and mitochondria as the major cellular targets of ...Continue Reading

References

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Citations

Nov 5, 1999·Journal of Applied Toxicology : JAT·V FessardJ M Poul
Jul 25, 2000·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M Strmac, T Braunbeck
Jul 16, 2014·Environmental Science and Pollution Research International·S Abdul MajeedA S Sahul Hameed
May 3, 2012·Chembiochem : a European Journal of Chemical Biology·Jacques LuxJean-François Nicoud
Aug 3, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Annalaura StammatiMauro Dacasto
Apr 12, 2013·Bioconjugate Chemistry·Matthew J SaundersWilson S Meng
Aug 15, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ryoko M UdaKeita Hayashi

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