Cytotoxic lymphocyte microRNAs as prospective biomarkers for Chronic Fatigue Syndrome/Myalgic Encephalomyelitis

Journal of Affective Disorders
Ekua W BrenuSonya M Marshall-Gradisnik

Abstract

Immune dysfunction associated with a disease often has a molecular basis. A novel group of molecules known as microRNAs (miRNAs) have been associated with suppression of translational processes involved in cellular development and proliferation, protein secretion, apoptosis, immune function and inflammatory processes. MicroRNAs may be implicated in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME), where immune function is impaired. The objective of this study was to determine the association between miRNAs in cytotoxic cells and CFS/ME. Natural Killer (NK) and CD8(+)T cells were preferentially isolated from peripheral blood mononuclear cells from all participants (CFS/ME, n=28; mean age=41.8±9.6 years and controls, n=28; mean age=45.3±11.7 years), via negative cell enrichment. Following total RNA extraction and subsequent synthesis of cDNA, reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) was used to determine the expression levels of nineteen miRNAs. There was a significant reduction in the expression levels of miR-21, in both the NK and CD8(+)T cells in the CFS/ME sufferers. Additionally, the expression of miR-17-5p, miR-10a, miR-103, miR-152, miR-146a, miR-106, miR-223 and miR-191 was signifi...Continue Reading

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Citations

May 21, 2013·Molecular Cancer·Yue ChenZhen-Ning Wang
Dec 18, 2013·International Immunology·Ekua Weba BrenuSonya M Marshall-Gradisnik
Jan 28, 2015·Toxicology and Applied Pharmacology·Xuefeng RenHongmei Wu
Sep 30, 2014·Rheumatology International·Jan L BjersingKaisa Mannerkorpi
May 6, 2014·Frontiers in Genetics·Neha Nagpal, Ritu Kulshreshtha
Jun 3, 2015·BMC Immunology·Sharni Lee HardcastleSonya Marshall-Gradisnik
Aug 5, 2018·The Journal of Pharmacology and Experimental Therapeutics·Erifili HatziagelakiTheoharis C Theoharides
Jul 10, 2019·Pain Practice : the Official Journal of World Institute of Pain·Hadeel A Al-RawafSami A Gabr
Dec 1, 2013·Auto- Immunity Highlights·E W BrenuS M Marshall-Gradisnik
Apr 20, 2017·BioMed Research International·Changzhuan ShaoAiping Chi
Jul 25, 2019·Diagnostics·Daniel MissailidisPaul R Fisher

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