Cytotoxic T cell response against lymphoblasts infected with Moloney (Abelson) murine leukemia virus. Methodological aspects and H-2 requirements

European Journal of Immunology
M J StukartC J Melitef

Abstract

A method for infection of lymphocytes with Moloney(Abelson) murine leukemia virus [M(A)-MuLV] is described. Only lymphoblasts obtained after stimulation of normal spleen cells by the B cell mitogen lipopolysaccharide (LPS) were satisfactory targets for virus-specific, secondary cytotoxic T lymphocytes (CTL), whereas spleen cells stimulated by the T cell mitogen concanavalin A were not. The secondary CTL response against M(A)-MuLV could be efficiently measured using M(A)-MulV-infected LPS blasts as stimulating cells for secondary in vitro restimulation and as target cells for virus-specific destruction. Cold target inhibition demonstrated virus specificity of CTL. The T cell character of the cytotoxic cells was demonstrated by their sensitivity to anti-Thy-1.2 treatment. Using syngeneic virus-infected LPS blasts as target and stimulator, CTL responses were measured with effector cells from C57BL mice of the H-2b haplotype and of recombinant haplotypes sharing either K or D alleles with H-2b. In analogy with previous studies on Moloney virus-specific CTL, it was observed that C57BL/6 (H-2b) effector cells predominantly lysed Db-compatible, virus-infected target cells; B10.A(5R), (KbDd) effector cells showed a poor CTL response ag...Continue Reading

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Citations

Nov 1, 1996·The Journal of Experimental Medicine·M E van den BroekH Hengartner
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Koen SchepersTon N M Schumacher
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T van HallR Offringa
Feb 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N J BeekmanF Ossendorp

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