Cytotoxic T cell responses to haptenated cells. III. Isolation and specificity analysis of continuously growing clones

European Journal of Immunology
W HaasH von Boehmer

Abstract

Various procedures were used to derive continuously growing cytotoxic T lymphocyte (CTL) clones from a primary culture containing responder cells from immunized mice and 3-(p-sulfophenyldiazo)-4-hydroxylphenyl acetic acid (SP)- or fluorescein isothiocyanate (FL)-coupled stimulator cells. It seems likely that CTL have to undergo some change, possibly genetic, to be able to grow continuously in T cell growth factor conditioned medium in the absence of any stimulator or filler cells. The most convenient and reliable procedure to generate CTL clones with different specificities was to establish from several aliquots of a primary culture cell populations continuously growing in medium conditioned with T cell growth factor(s). Clones with different specificities segregated in the different populations. SP- and FL-specific CTL clones restricted to H-2Kk and H-2Dd and two FL-specific CTL clones with no apparent H-2 restriction are described.

References

Aug 1, 1979·European Journal of Immunology·H von BoehmerW Haas
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M J Bevan
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May 1, 1980·Springer Seminars in Immunopathology·H von BoehmerM Nabholz
Apr 1, 1980·The Journal of Experimental Medicine·A L Glasebrook, F W Fitch

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Citations

Jan 1, 1982·Immunogenetics·K S ZierG R Pierson
Jun 1, 1985·Die Naturwissenschaften·I ZieglerW J Müller
Jul 30, 1982·Journal of Immunological Methods·W Haas, H Von Boehmer
Jan 1, 1984·Human Immunology·R B Effros, R L Walford
Jan 1, 1989·Experimental Gerontology·N L PerilloR B Effros
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R Palacios
Jan 1, 1982·The Journal of Experimental Medicine·Y Kurosawa, S Tonegawa
Jun 1, 1982·The Journal of Experimental Medicine·D H Raulet, M J Bevan
Oct 1, 1983·The Journal of Experimental Medicine·T A PotterT V Rajan
Mar 1, 1988·Molecular and Cellular Probes·R I Lechler
Dec 1, 1981·Scandinavian Journal of Immunology·K F Lindahl, J Langhorne
Jan 1, 1988·European Journal of Immunology·G De LiberoS H Kaufmann
Jun 1, 1987·European Journal of Immunology·M NabholzR Gabathuler
Jan 1, 1986·European Journal of Immunology·R Palacios, H Von Boehmer
Aug 1, 1985·European Journal of Immunology·W Haas, P Kisielow
Oct 1, 1982·European Journal of Immunology·H S TehH Von Boehmer
Aug 1, 1985·European Journal of Immunology·W Haas, P Kisielow
Mar 1, 1982·European Journal of Immunology·J GüntherH Von Boehmer
Apr 1, 1987·European Journal of Immunology·W Müller, P Vandenabeele
Oct 1, 1982·European Journal of Immunology·H Von BoehmerJ Langhorne
May 1, 1984·Journal of Virology·H C Ertl, R W Finberg
Mar 1, 1986·Microbiological Reviews·F W Fitch

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