PMID: 6972538Apr 1, 1981Paper

Cytotoxic T lymphocyte hybridomas that mediate specific tumor-cell lysis in vitro

Proceedings of the National Academy of Sciences of the United States of America
Y KaufmannZ Eshhar

Abstract

Cytotoxic hybridomas were generated by polyethylene glycol-induced fusion of cytotoxic T lymphocytes (CTL) and BW5147 lymphoma cells. The CTL populations used for fusion were obtained from BALB/c (H-2d) mice primed with leukemia EL4 of C57BL/6 (H-2b) and restimulated either in vivo or in vitro. To circumvent possible CTL-mediated nonspecific lysis of BW5147 cells during fusion, the CTL were transiently inactivated by trypsin prior to fusion. Four cytolytically active hybridomas were obtained, cloned, and subcloned. Hybrid clones lysed all H-2b leukemic target cells tested but not lipopolysaccharide- or concavanalin A-stimulated C57BL/6 lymphoblasts or non-H2b target tumor cells. The mechanism of hybridoma-mediated killing of target cells in vitro appears to be similar to that of parental CTL, although some differences have been observed. The hybridomas appear to possess neither natural killing nor antibody-dependent cytolytic activity. Clones of hybrids propagated in culture for over 6 months without the addition of known external stimulus (i.e., independent of cell growth factor and antigen) exhibit specific lytic activity against H-2b tumor cells. Such autonomous hybridomas will provide a tool for studying the mechanism of CT...Continue Reading

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Citations

Jul 30, 1982·Journal of Immunological Methods·W Haas, H Von Boehmer
Feb 19, 1983·Lancet·D H CrawfordE R Huehns
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J LeW Prensky
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S M HedrickY Kaufmann
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Jun 18, 2016·Journal of Immunology Research·John Reiser, Arnob Banerjee
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Feb 6, 2016·Journal of Immunotherapy·Hadas Weinstein-MaromGideon Gross

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