Cytotoxic T-lymphocyte responses in cattle infected with bovine viral diarrhea virus

Veterinary Microbiology
M BeerO R Kaaden

Abstract

A system for a reproducible in vitro restimulation of bovine viral diarrhea virus (BVDV)-specific cytotoxic T-cells (CTL) was developed. Lymphocyte cultures of BVDV-immunised cattle were stimulated with infectious BVDV isolate PT810 and recombinant bovine interleukin-2 for 12 to 25 days. A specific lysis of Concanavalin A-stimulated BVDV-infected autologous target cells was observed, whereas allogeneic BVDV-infected target cells were only marginally lysed as detected by flow cytometry. BVDV-specific lymphocyte transformation was further characterised by the expression of bovine lymphocyte activation antigens and bovine MHC class-II molecules. Secondary stimulation of CTL was influenced by in vitro production of BVDV-specific neutralising antibodies, which were secreted exclusively in BVDV-inoculated lymphocyte cultures of immunised cattle. These results demonstrate the presence of CTL in peripheral blood mononuclear cells (PBMC) of immunised cattle which can kill autologous BVDV-infected antigen-presenting cells after in vitro restimulation.

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Citations

Apr 25, 2000·Virus Research·T Collen, W I Morrison
Mar 26, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M BeerO R Kaaden
Apr 14, 2005·Journal of Virology·Sascha TrappNikolaus Osterrieder
Dec 18, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·H-R FreyV Moennig
Sep 17, 2005·Animal Health Research Reviews·Volker MoennigAnn Lindberg
Oct 31, 2009·Veterinary Microbiology·Johanna ZemkeMartin Beer
Jul 22, 2016·Heliyon·Jörn GethmannFranz J Conraths
Oct 13, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S KuhneA Ballagi
Jan 15, 2008·Veterinary Immunology and Immunopathology·Ratree PlattJames A Roth
Oct 6, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Elisabeth M Liebler-TenorioJohn D Neill
Oct 13, 2009·European Journal of Nuclear Medicine and Molecular Imaging·Ya-Yao HuangChyng-Yann Shiue
Dec 8, 2020·Frontiers in Immunology·Neha SangewarWaithaka Mwangi
Mar 26, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neha SangewarWaithaka Mwangi
Jan 1, 2005·Veterinary Immunology and Immunopathology·Ronald M SilflowAllen G Harmsen

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