PMID: 9525615Apr 3, 1998Paper

Cytotoxic T-lymphocyte target proteins and their major histocompatibility complex class I restriction in response to adenovirus vectors delivered to mouse liver

Journal of Virology
K JoossJ M Wilson

Abstract

The activation of cytotoxic T lymphocytes (CTLs) to cells infected with adenovirus vectors contributes to problems of inflammation and transient gene expression that attend their use in gene therapy. The goal of this study was to identify in a murine model of liver gene therapy the proteins that provide targets to CTLs and to characterize the major histocompatibility complex (MHC) class I restricting elements. Mice of different MHC haplotypes were infected with an E1-deleted adenovirus expressing human alkaline phosphatase (ALP) or beta-galactosidase as a reporter protein, and splenocytes were harvested for in vitro CTL assays to aid in the characterization of CTL epitopes. A library of vaccinia viruses was created to express individual viral open reading frames, as well as the ALP and lacZ transgenes. The MHC haplotype had a dramatic impact on the distribution of CTL targets: in C57BL/6 mice, the hexon protein presented by both H-2Kb and H2Db was dominant, and in C3H mice, H-2Dk-restricted presentation of ALP was dominant. Adoptive transfer of CTLs specific for various adenovirus proteins or transgene products into either Rag-I or C3H-scid mice infected previously with an E1-deleted adenovirus verified the in vivo relevance of...Continue Reading

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Citations

Jul 23, 2002·Human Gene Therapy·Melanie OlivePhyllis Flomenberg
Dec 4, 2003·Journal of Virology·Teng Chih YangJonathan Bramson
Dec 18, 2008·The Journal of General Virology·Amita JoshiPhyllis Flomenberg
Sep 12, 2013·Reviews in Medical Virology·Sangeetha HareendranGiridhara R Jayandharan
Jul 14, 2004·Medicinal Research Reviews·Hai-sheng ZhouChih-chuan Liang
Feb 17, 2005·Pancreas·Alexander L ShifrinSteven E Raper
Mar 8, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Z X LiuG Dennert
Oct 19, 2005·Gene Therapy·T B FerreiraM J T Carrondo
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Jul 19, 2003·Human Gene Therapy·Eva Maria Borst, Martin Messerle
Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jong Myun ParkJay A Berzofsky
Nov 27, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Senthil Kumar VenugopalMark A Zern
Jun 3, 2009·Expert Review of Vaccines·Erin E ThackerQiana L Matthews
May 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuejin LiangJiaren Sun
Apr 5, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jianlin GengZhigang Tian
Oct 20, 2000·The Journal of General Virology·W C Russell
Dec 12, 2001·Journal of Virology·Valérie Molinier-FrenkelJean-Gérard Guillet
May 7, 2002·Journal of Virology·Andrei N VarnavskiJames M Wilson
Feb 19, 2000·Microscopy Research and Technique·D Hartigan-O'Connor, J S Chamberlain
Mar 12, 2005·Human Gene Therapy·Dan H Barouch, Gary J Nabel

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