Cytotoxic T lymphocytes generated across and I-Ab mutant difference are directed against a molecule bearing Ia antigens

European Journal of Immunology
L P de WaalR W Melvold

Abstract

The recently discovered B6.C-H-2bm12 (bm12) histocompatibility mutant bears a mutation of the I-A subregion of the H-2b haplotype. We have studied cytotoxic T lymphocytes (CTL) generated in secondary mixed lymphocyte culture (MLC) between the bm12 mutant and the strain of origin C57BL/6 (B6). In both directions, strong CTL responses were generated against lipopolysaccharide-stimulated, but not against concanavalin A-stimulated target cells. Studies of the specificity of the bm12 anti-B6 CTL response, using target cells from a panel of H-2 recombinants, showed that the CTL were directed against specificities of the I-Ab subregion. This conclusion was confirmed by the observation that the bm12 anti-B6 CTL could be specifically blocked by anti-Iab, but not by anti-Kb antisera. EL4 tumor cells, which express Kb and Db but not Iab antigens, were not lysed by the CTL. The combined data confirm that bm12 is an I-A subregion mutant and indicate that the I-A subregion antigens, recognized by antibody and those recognized by CTL, are probably present on the same molecules. The CTL effector cells generated in secondary MLC across the I-Ab mutant difference had the surface phenotype Lyt 1- 2+.

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Citations

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·H Y TseT H Hansen
Aug 1, 1982·The Journal of Experimental Medicine·H Y LeiC Waltenbaugh
Mar 1, 1983·The Journal of Experimental Medicine·G M Shearer, R B Levy
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Jan 1, 1985·Critical Reviews in Clinical Laboratory Sciences·H Bril, R Benner
Mar 1, 1982·European Journal of Immunology·M S SandrinG J Hämmerling

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