Cytotoxicity and altered c-myc gene expression by medical polyacrylamide hydrogel

Journal of Biomedical Materials Research. Part a
T F XiL L Chou

Abstract

Medical Polyacrylamide Hydrogel (PAMG)has been used in plastic and aesthetic surgery for years. However, its safety is still in doubt in many countries. In the current research, first an approach, using high performance liquid chromatography (HPLC), to determine the amount of residual acrylamide monomer (AM) in the PAMG was presented. Then the cytotoxicity of PAMG was investigated using cell counting and methyl thiazolyl tetrazolium (MTT) assay. To explore the mechanism of this toxicity, normal human fibroblasts cultured in medium extracts were analyzed. Membrane changes and other related parameters were investigated using flow cytometry (FCM). Real time fluorescent polymerase chain reaction (real time PCR) was also introduced to determine the biological response of the fibroblasts. During this process, three representative genes (p53, beta-actin, and c-myc, which are tumor suppressor genes, housekeeping genes, and proto-oncogenes respectively) were selected for examination. Results indicated that a method based on HPLC is practical and simple for determining AM in PAMG. The detection limits can reach the desired ppb level, and so it can fully meet the requirements of the studies of PAMG. Polyacylamide Hydrogel inhibits the gro...Continue Reading

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Citations

Jan 22, 2009·Aesthetic Plastic Surgery·Ning-Xin ChengShi-Liang Xu
Jul 12, 2008·Annals of Biomedical Engineering·Frank Xue JiangNoshir A Langrana
Jan 17, 2012·ACS Applied Materials & Interfaces·Jiantao LinLi-Ming Zhang
Dec 30, 2017·The Breast Journal·Lance S EbersonMarisa H Borders
Oct 10, 2009·Biotechnology and Bioengineering·Stephanie NemirJennifer L West
Jul 22, 2010·Angewandte Chemie·Katrin KnopUlrich S Schubert
Mar 22, 2016·Molecular and Clinical Oncology·Gang ChenJin-Long Huang
Aug 21, 2021·Plastic and Reconstructive Surgery. Global Open·Shigeki SakaiKazuo Kishi
Oct 13, 2011·Acta Biomaterialia·Peng-Yuan WangNicolas H Voelcker

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