Abstract
Oldenlandia diffusa is a traditional Chinese herbal remedy with known cytotoxic activity in vitro and in vivo. The aim of the study was to identify the most cytotoxic constituents of a water extract (a decoction is traditionally used as a treatment) by HPLC and activity-guided fractionation. The bioavailability of the decoction and certain fractions, and the mode of cell death induced by these mixtures, were also investigated. A decoction of O. diffusa was prepared and separated by HPLC into eleven fractions (F1-11) for testing on the growth of HL60 leukaemia cells; two of the most active fractions were also tested on Caco-2 colon cancer cells. Cell viability was measured by trypan blue exclusion, DNA content (Cyquant NF assay) and neutral red uptake. Evidence of apoptosis was gained from cells stained with the nuclear dye DAPI, and detection of cleaved poly (ADP-ribose) polymerase (PARP) by Western blot. Fraction 9 was found to be the most active fraction, and, along with the decoction, induced apoptosis. Cells stained with DAPI showed a decrease in cell size and nuclear fragmentation characteristic of apoptosis. Detection of cleaved PARP further confirmed induction of apoptosis by O. diffusa decoction and fraction 9. The bioa...Continue Reading
References
Jul 6, 1995·Nature·D W NicholsonY A Lazebnik
May 26, 1998·The EMBO Journal·Q L DeverauxJ C Reed
Jul 1, 1997·International Journal of Immunopharmacology·Y YoshidaU Yamashita
May 12, 2000·Journal of Clinical Pharmacology·J T Cheng
Feb 1, 1996·Cancer Biotherapy & Radiopharmaceuticals·B Y WongC P Wan
Mar 27, 2002·Cancer Chemotherapy and Pharmacology·David SadavaSusan E Kane
Sep 17, 2002·Biological & Pharmaceutical Bulletin·Hwan-Suck ChungHyung-Min Kim
Aug 9, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Isabel Behrens, Thomas Kissel
Jul 27, 2004·Journal of Herbal Pharmacotherapy·Salin GuptaJun Shao
Jun 1, 2005·European Journal of Medicinal Chemistry·Chao-Mei MaMohsen Daneshtalab
Feb 24, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·R C R BeckC M Lehr
Oct 16, 2007·Journal of Ethnopharmacology·Shaun WillimottElizabeth I Opara
Mar 15, 2008·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Zhitao LiangZhongzhen Zhao
May 13, 2009·Life Sciences·Pui Kei WuWen Luan Wendy Hsiao
Citations
Dec 24, 2013·European Journal of Medicinal Chemistry·Bianka SiewertRené Csuk
Jul 26, 2014·Journal of Pharmaceutical and Biomedical Analysis·Cunman LiXinmiao Liang
Jan 26, 2016·Pharmaceutical Biology·Roman PaduchJan Fiedurek
Aug 13, 2011·Journal of Pharmaceutical and Biomedical Analysis·Tao Zhu, Kyung Ho Row
Jul 1, 2011·Journal of Biomedicine & Biotechnology·Xuemei WangXiulan Su
Aug 21, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Fan CheungYibin Feng
Apr 17, 2012·Biochimica Et Biophysica Acta·Bozena Pawlikowska-PawlegaWiesław I Gruszecki
Oct 24, 2012·Biochimica Et Biophysica Acta·Bożena Pawlikowska-PawlęgaWiesław I Gruszecki
Sep 5, 2013·Biochimica Et Biophysica Acta·Bożena Pawlikowska-PawlęgaWieslaw I Gruszecki
May 13, 2014·Biochimica Et Biophysica Acta·Bożena Pawlikowska-PawlęgaWieslaw I Gruszecki
Apr 9, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yun-Young SunwooSang In Park
Sep 6, 2012·International Journal of Biological Macromolecules·Adrian WiaterJanusz Szczodrak
Mar 10, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Niege A J C FurtadoChristelle M André
Jan 4, 2018·Chinese Medicine·Min DaiHongzhi Yang
Feb 18, 2012·Pharmaceutical Biology·Anna Woźniak, Roman Paduch
Feb 26, 2016·Oncotarget·Sahdeo PrasadBharat B Aggarwal
Mar 23, 2013·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Anna SzakielChristophe Bertsch
Jul 18, 2012·Journal of Immunotoxicology·V Vinod Prabhu, C Guruvayoorappan
Jun 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui ChenMenghua Liu
Oct 12, 2019·Biomolecules·Yanan SongTonghua Liu
Jul 10, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jinxia LiLi Shen
Jul 28, 2019·Pharmaceutics·Amélia M SilvaEliana B Souto
Jun 19, 2019·Bioorganic Chemistry·Gianfranco FontanaSergio Rosselli