Cytotoxicity and DNA interaction of brucine and strychnine-Two alkaloids of semen strychni

International Journal of Biological Macromolecules
Fei LiuWeijun Kang

Abstract

The cytotoxicities of the two alkaloids strychnine and brucine from the seed of Strychnos nux-vomica and their interaction with DNA were investigated. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrasolium bromide (MTT) assay was used to examine the growth inhibitory effects of these alkaloids on Vero cells after 24, 48 and 72h of incubation. The cytotoxicities of strychnine and brucine were found to be time- and concentration-dependent. Strychnine was determined to be more toxic to Vero cells than brucine. At the same time, the interactions of strychnine and brucine with DNA were investigated using neutral red (NR) dye as a probe by UV-vis spectroscopy, fluorescence spectroscopy, and an examination of the ionic strength effect, and the effects of alkaloids on DNA melting were also examined. The results indicated that a DNA-brucine mixture but not a DNA-strychnine mixture could be extracted from Vero cells after treatment with brucine and strychnine, respectively. Brucine competitively intercalated into the DNA double-helix causing fluorescence quenching of the DNA-NR system. UV absorption spectroscopy and the melting temperature (Tm) curve also provided evidence that brucine interacted with DNA through intercalation. Furtherm...Continue Reading

References

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Aug 11, 2004·Toxicon : Official Journal of the International Society on Toxinology·Geneviève PhilippeMichel Frédérich
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Feb 8, 2011·Toxicology·Dennis Mulac, Hans-Ulrich Humpf

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Citations

Jan 5, 2018·The American Journal of Chinese Medicine·Rixin GuoZhuju Wang
Apr 21, 2020·Frontiers in Pharmacology·Lu LuXin Luan
Dec 14, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Li-Li LiuLi-Hua Chen
Jan 9, 2021·The American Journal of Chinese Medicine·Jun-Yan XiangQiuhong Xie
Apr 2, 2021·The Journal of Pharmacy and Pharmacology·Lu GaoBaojian Wu

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