Cytotoxicity and genotoxicity of natural resin-based experimental endodontic sealers

Clinical Oral Investigations
Gleyce O SilvaCarlos Henrique Ribeiro Camargo

Abstract

The development of endodontic sealers based on natural resins seems to be promising, given their improved biological properties. This study evaluated the cytotoxic and genotoxic effects of two experimental root canal sealers, based on extracts from Copaifera multijuga and Ricinus communis (castor oil polymer), comparing them to synthetic resin-based sealers: a single methacrylate-based, a multi-methacrylate-based, and an epoxy resin-based sealers. Sealers were prepared, set, and exposed to cell culture medium for 24 h at 37 °C with CO2. V79 cells were exposed to serial dilutions of the extracts of each sealer for 24 h. Cell viability was measured by the MTT assay and genotoxicity was assessed by the formation of micronuclei. The single methacrylate-based sealer had the most cytotoxic effects, with significant reduction in cell viability in all dilutions of the extract. The castor oil polymer-based sealer was, on the other hand, the most biocompatible sealer, with no cytotoxic effects at any concentration. All tested sealers were not genotoxic, excepting the single methacrylate-based sealer. The tested natural resin-based sealers presented low cytotoxic and no genotoxic effects on cell cultures. These results may suggest a good ...Continue Reading

References

Mar 13, 2003·Journal of Oral Rehabilitation·M Dartar OztanL Zaimoğlu
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Valdemar M R BarrosGilberto Chierice
Nov 5, 2003·Phytotherapy Research : PTR·Sylvia R M LimaPatricia D Fernandes
Mar 29, 2005·Journal of Endodontics·Ivana MiletićMaja Osmak
Oct 4, 2005·Dental Materials : Official Publication of the Academy of Dental Materials·J VolkW Geurtsen
Nov 30, 2005·Journal of Oral Science·Ardo SabirWihaskoro Sosroseno
Dec 20, 2005·Mutation Research·Carla R Andrighetti-FröhnerCláudia M O Simões
Sep 20, 2006·Journal of Endodontics·Serge BouillaguetPetra E Lockwood
Sep 26, 2006·Journal of Dental Research·H SchweiklG Schmalz
Jun 12, 2008·Memórias do Instituto Oswaldo Cruz·Adriana Oliveira Dos SantosCelso Vataru Nakamura
Oct 25, 2008·Brazilian Dental Journal·Paulo Tadeu da SilvaRenato Toledo Leonardo
Jan 10, 2009·International Endodontic Journal·M G BrackettJ C Wataha
Sep 25, 2009·Acta histochemica·Nimet GencogluDilek Helvacioglu
Nov 17, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Carlos Henrique Ribeiro CamargoHelmut Schweikl
Nov 26, 2009·Journal of Applied Oral Science : Revista FOB·Gabriela Tavares Bekner CorreaMiriam F Zaccaro Scelza
Jan 19, 2010·International Endodontic Journal·A S Al-HiyasatH Darmani
Dec 18, 2010·Mutagenesis·Yun HuangQinghua Shi
Feb 11, 2011·International Endodontic Journal·M Karapınar-KazandağG Bayırlı

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Citations

Aug 9, 2020·Clinical Oral Investigations·Fábio Miguel Dos Santos CostaSilvia Regina Batistuzzo de Medeiros
Mar 23, 2020·Odontology·Ana Beatriz Vilela TeixeiraAndréa Cândido Dos Reis
Apr 2, 2021·Phytotherapy Research : PTR·Diorge Jônatas Marmitt, Mohamad Hesam Shahrajabian

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