PMID: 11309795Apr 20, 2001Paper

Cytotoxicity and immunogenicity of SACCHACHITIN and its mechanism of action on skin wound healing

Journal of Biomedical Materials Research
W S HungY H Tsai

Abstract

SACCHACHITIN membrane, a weavable skin substitute made from the residual fruiting body of Ganoderma tsugae, has been demonstrated to promote skin wound healing. Prior to its clinical application, it is critical to learn more about any possible cytotoxicity, immunogenicity, or allergy response, and at least some of its mechanism(s) of action(s). In the present studies, it has been found that SACCHACHITIN suspension at less than 0.05% shows no cytotoxicity to the primary culture of rat fibroblasts. However, at higher concentrations (> or = 0.1%), it does reduce the growth of fibroblasts, based on MTT assays. This might be caused by positive charges on chitin molecules that are too strong, and may be harmful to the cell membrane. SACCHACHITIN showed no immunogenicity after it was inoculated into rats three times; however, the unmodified, purified rabbit type I and type II collagens did. Subcutaneous injection of SACCHACHITIN suspension into rats showed no gross allergic responses on skin. Nevertheless, it did cause local acute inflammation, as observed by histological investigation. This is similar to what occurred in the wound site covered with SACCHACHITIN membrane. The chemotactic effect of SACCHACHITIN was exhibited in both in...Continue Reading

Citations

Apr 18, 2003·Biomaterials·Eugene Khor, Lee Yong Lim
Jul 17, 2004·Journal of Biomaterials Science. Polymer Edition·Zarana S Patel, Antonios G Mikos
Sep 30, 2017·The Journal of Pharmacy and Pharmacology·Mario MarescaLorenzo Di Cesare Mannelli
Dec 24, 2014·Journal of Basic and Clinical Physiology and Pharmacology·Asie ShojaiiNazanin Rahnama
Jan 22, 2015·International Journal of Molecular Medicine·Lei QiuYan Zhao
May 7, 2008·Mycopathologia·Chih-Ching ChienChing-Hui Tseng
Oct 20, 2018·Frontiers in Bioengineering and Biotechnology·Giulia SuaratoAthanassia Athanassiou
Oct 1, 2014·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Deepa NarayananK P Chennazhi
Sep 17, 2005·Journal of Materials Science. Materials in Medicine·A P MarquesR L Reis
May 29, 2007·Journal of Biomedical Materials Research. Part a·Mark J Butler, Michael V Sefton
Jun 17, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Wei-Sheng HungYu-Hui Tsai
Aug 12, 2017·International Wound Journal·Rita SinghAntaryami Singh

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